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anther - specific cdna - sequences, genomic dna sequences - and - a recombinant dna sequences

机译:花药特异性cdna序列,基因组dna序列以及重组dna序列

摘要

cDNA sequences are disclosed which are expressed specifically in the anther of a plant. Genomic DNA sequences corresponding to the cDNA clones are obtained using the cDNA clones as hybridization probes. Recombinant, or chimeric, DNA sequences are constructed in which the promoter sequence from anther-specific genomic clones are operatively linked to a DNA sequence coding for a desired polypeptide. Transgenic plants are made in which the chimeric DNA sequences are expressed in the anther of the transgenic plant. In a preferred embodiment, the coding DNA sequence expresses a polypeptide which will disrupt formation of viable pollen, resulting in a male-sterile plant.
机译:公开了在植物的花药中特异性表达的cDNA序列。使用cDNA克隆作为杂交探针获得对应于cDNA克隆的基因组DNA序列。构建重组或嵌合的DNA序列,其中来自花药特异性基因组克隆的启动子序列与编码所需多肽的DNA序列可操作地连接。制备其中嵌合DNA序列在转基因植物的花药中表达的转基因植物。在一个优选的实施方案中,编码DNA序列表达将破坏活花粉形成的多肽,从而产生雄性不育植物。

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