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METHOD FOR ISOLATING CHROMOSOMAL DNA FROM BACTERIAL CELLS OF PLAGUE PATHOGEN, YERSINIA PESTIS
METHOD FOR ISOLATING CHROMOSOMAL DNA FROM BACTERIAL CELLS OF PLAGUE PATHOGEN, YERSINIA PESTIS
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机译：从鼠疫耶尔森菌的细菌细胞中分离染色体DNA的方法
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摘要
FIELD: biotechnology, microbiology, preparative biochemistry.;SUBSTANCE: invention can be used for isolation of chromosomal DNA from bacterial cells. Cell culture of Yersinia pestis is cultured on solid nutrient medium, inactivated with chloroform and washed off from medium surface. The lysing substance Triton X-100 is added to prepared cell suspension to its final concentration 1% and incubated at temperature +37 C for 1 h. Then DNA is purified from cellular residual debris and centrifugation is carried out in cesium chloride density gradient with ethidium bromide. Method provides preparing highly purified chromosomal DNA of plague pathogen in preparative amount and with prolonged time storing..;EFFECT: improved isolating method.;3 ex
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