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METHOD FOR QUANTITATIVE EVALUATION OF FUNCTIONAL ACTIVITY OF CUTANEOUS MAST CELLS
METHOD FOR QUANTITATIVE EVALUATION OF FUNCTIONAL ACTIVITY OF CUTANEOUS MAST CELLS
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机译:皮肤肥大细胞功能活性的定量评估方法
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FIELD: experimental medicine. SUBSTANCE: a dermal tissue sample should be reduced, trypsinized, thermostatted, centrifuged; a control sample is prepared by adding physiological solution, and a comparison sample is prepared due to adding solution of degranulation activator to be thermostatted, centrifuged, dyed with 0.3%-neutral red solution to carry out photocolorimetry at 490 nm, then mast cells degranulation degree(MCDD) is calculated, and at MCDD values ranged 2.1-2.5 functional activity of mast cells corresponds to norm. EFFECT: higher accuracy of evaluation. 1 tbl
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