首页> 外国专利> DEDICATED PLIPEPTID, METHOD FOR ITS PRODUCTION, method of amplifying DNA, cDNA cloning techniques SECOND AND DNA sequencing method for labeling DNA METHOD reverse transcription of DNA sequencing methods

DEDICATED PLIPEPTID, METHOD FOR ITS PRODUCTION, method of amplifying DNA, cDNA cloning techniques SECOND AND DNA sequencing method for labeling DNA METHOD reverse transcription of DNA sequencing methods

机译：专用肽，其生产方法，DNA扩增方法，cDNA克隆技术标记DNA的第二种方法和DNA测序方法DNA测序方法的反转录

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FIELD: biotechnology.;SUBSTANCE: invention relates to method for DNA amplification, method for cloning of second DNA and method for DNA reverse transcription. In each method heat stable DNA polymerase is used which encoded by SEQ ID NO:7, derived from Anaerocellum thermophilum or recombinant R.coli strain, transformed by vector containing SEQ ID NO:7. DNA polymerase catalyzes matrix-targeted DNA polymerization, has 5'-3'-activity and reverse transcriptase activity, and has no 3'-5'-endonuclease activity in presence of magnesium ions and in absence of manganese ions. DNA polymerase retains at least 90 % of its activity after incubation for 30 min at 80C in absence of stabilizing detergents and has apparent molecular mass between of approximately 96 kDa and approximately 100 kDa. Magnesium-dependent reverse transcriptase activity of DNA polymerase is more than 30 % of DNA polymerase activity, and manganese-dependent reverse transcriptase activity of said polypeptide is more than 60 % of DNA polymerase activity.;EFFECT: increased precision of matrix RNA transcrption at high temperatures.;7 ck, 5 dwg, 7 ex

机译：技术领域：本发明涉及DNA扩增的方法，第二DNA的克隆的方法和DNA反转录的方法。在每种方法中，都使用热稳定的DNA聚合酶，该酶由SEQ ID NO：7编码，该酶衍生自嗜热厌氧杆菌或重组R.coli菌株，并经含有SEQ ID NO：7的载体转化。 DNA聚合酶催化以基质为靶标的DNA聚合，具有5'-3'活性和逆转录酶活性，并且在存在镁离子和不存在锰离子的情况下不具有3'-5'核酸内切酶活性。在没有稳定去污剂的情况下，DNA聚合酶在80°C下孵育30分钟后，其活性至少保留90％，其表观分子量约为96 kDa至100 kDa。 DNA聚合酶的镁依赖性逆转录酶活性超过DNA聚合酶活性的30％以上，所述多肽的锰依赖性逆转录酶活性超过DNA聚合酶活性的60％以上;效果：在高温下提高基质RNA转录的精度温度。; 7 ck，5 dwg，7 ex

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公开/公告号RU2002114591A

专利类型
公开/公告日2004-02-20

原文格式PDF
申请/专利权人 РОШЕ ДИАГНОСТИКС ГМБХ (DE);
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申请/专利号RU20020114591
发明设计人 АНКЕНБАУЕР ВАЛЬТРАУД (DE);ШМИТЦ-АГХЕГУИАН ГУДРУН (DE);БОНЧ-ОСМОЛОВСКАЯ ЕЛИЗАВЕТА (RU);СВЕТЛИЧНЫЙ ВИТАЛИЙ (DE);МАРКАУ УРСУЛА (DE);АНГЕРЕР БЕРНХАРД (DE);РАЙСЕР АСТРИД (DE);
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申请日1997-10-01
分类号C12N15/65;C12N15/09;C12N15/10;
国家 RU
入库时间 2022-08-21 22:45:40

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