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ASCORBATE PEROXIDASE FROM CAPSICUM ANNUUM L. CV. HANBYUL INDUCED BY PLANT PATHOGENS, GENES ENCODING THE SAME AND METHOD FOR DETECTING PLANT DEFENSE RESPONSE USING THE SAME GENES
ASCORBATE PEROXIDASE FROM CAPSICUM ANNUUM L. CV. HANBYUL INDUCED BY PLANT PATHOGENS, GENES ENCODING THE SAME AND METHOD FOR DETECTING PLANT DEFENSE RESPONSE USING THE SAME GENES
PURPOSE: Ascorbate peroxidase from Capsicum annuum L. cv. Hanbyul induced by plant pathogens, genes encoding the same and a method for detecting plant defense response using the same genes are provided, which genes are useful as markers for detecting plant defense response, and are used for producing plants having improved tolerance to plant pathogens. CONSTITUTION: The ascorbate peroxidase encoding genes from Capsicum annuum L. cv. Hanbyul induced by plant pathogens that is expressed by plant pathogens or plant disease tolerance inducing materials have the nucleotide sequences set forth in SEQ ID NO:1 and SEQ ID NO: 3, wherein the plant pathogen is one selected from Xanthomonas campestris pv. vesicatoria, Phytophthora capsici and Colletorichum gloeosporioides; and the plant disease tolerance inducing material is salicylic acid or hydroperoxide. The method for detecting plant defense response comprises the steps of: infecting a plant with Xanthomonas campestris pv. vesicatoria; detecting expression of genes encoding ascorbate peroxidase or peroxidase; and measuring the activity of total peroxidase and accumulated amount of hydroperoxide.
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机译：目的：来自辣椒辣椒的抗坏血酸过氧化物酶。提供了由植物病原体诱导的Hanbyul，编码该基因的基因以及使用相同基因检测植物防御反应的方法，该基因可用作检测植物防御反应的标记，并用于产生对植物病原体的耐受性提高的植物。组成：辣椒中抗坏血酸过氧化物酶编码基因。由植物病原体或植物抗病诱导材料表达的由植物病原体诱导的Hanbyul具有SEQ ID NO：1和SEQ ID NO：3所示的核苷酸序列，其中植物病原体选自野油菜黄单胞菌（Panthomonas campestris pv）。 vesicatoria，Phytophthora capsici和Colletorichum gloeosporioides;植物抗病诱导物质为水杨酸或氢过氧化物。用于检测植物防御反应的方法包括以下步骤：用黄单胞菌（Xanthomonas campestris pv）感染植物。 vesicatoria;检测编码抗坏血酸过氧化物酶或过氧化物酶的基因的表达;测定总过氧化物酶的活性和过氧化氢的累积量。
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