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A RAPID DNA EXTRACTION METHOD FOR PCR-BASED ANALYSIS OF TRANSGENIC FISH
A RAPID DNA EXTRACTION METHOD FOR PCR-BASED ANALYSIS OF TRANSGENIC FISH
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机译：基于PCR的转基因鱼快速DNA提取方法
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摘要
PURPOSE: A DNA extraction method for PCR analysis of a transgenic fish is provided, thereby rapidly extracting DNA for PCR analysis to analyze a transgenic fish rapidly and cheaply. CONSTITUTION: A DNA extraction method for PCR analysis of a transgenic fish comprises the steps of: culturing a tissue such as a fin sampled from a fish in a basic buffer solution containing sodium dodecyl sulfate(SDS), buffer solution-X at 90 deg. C for 10 minutes; adding a buffer solution containing a nonionic surfactant including NP 40(nonidet P-40; octylphenoxy polyethoxy ethanol) and Tween 20, buffer solution-Y into the cultured medium to neutralize the medium; and centrifuging the cultured medium and recovering the supernatant, wherein the buffer solution-X contains 20 mM NaOH, 1 mM EDTA and 0.01% SDS and has pH 11.0; and the buffer solution-Y contains 50 mM Tris-HCl, 2% Tween 20, 2% NP 40 and 5 mM MgCl2 and has pH 7.2.
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机译：目的：提供一种DNA提取方法，用于PCR分析转基因鱼，从而快速提取DNA用于PCR分析，从而快速，廉价地分析转基因鱼。组成：一种用于转基因鱼PCR分析的DNA提取方法，包括以下步骤：在含有十二烷基硫酸钠（SDS），缓冲液-X的基本缓冲液中于90度培养组织（例如从鱼中取样的鳍）。 C持续10分钟;将含有NP 40（壬基P-40;辛基苯氧基聚乙氧基乙醇）的非离子表面活性剂和吐温20的缓冲溶液-Y的缓冲溶液加入到培养基中以中和。离心分离培养液，回收上清液，缓冲液-X中含有20mM NaOH，1mM EDTA和0.01％SDS，pH为11.0。缓冲液-Y包含50 mM Tris-HCl，2％Tween 20、2％NP 40和5 mM MgCl2，pH值为7.2。
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