METHOD FOR PRODUCING TRANSGLUTAMINASE-CROSS-LINKED PROTEINS OF VEGETABLE ORIGIN, PROTEIN GELS AND THE USE THEREOF

• 1. 一种蛋白酶水解耦合转谷氨酰胺酶交联修饰制备低致敏性乳清蛋白的方法 [P] . 中国专利： CN108902443A . 2018-11-30
• 2. 获得单体形式的重组凝血酶原激活蛋白酶 ( r L O P A P )的方法；重组凝血酶原激活蛋白酶 ( r L O P A P )及其氨基酸序列；这种蛋白酶作为去纤维蛋白原酶制剂以及凝血酶原血异常诊断试剂盒的用途 [P] . 中国专利： CN101068831A . 2007-11-07
• 3. Method for producing transglutaminase-cross-linked proteins of vegetable origin, protein gels and the use thereof [P] . 美国专利： US2004241284A1 . 2004-12-02

  机译： 植物来源的转谷氨酰胺酶交联蛋白的生产方法，蛋白凝胶及其用途

• 4. METHOD FOR PRODUCING TRANSGLUTAMINASE-CROSS-LINKED PROTEINS OF VEGETABLE ORIGIN, PROTEIN GELS AND THE USE THEREOF [P] . CA2457132A1 . 2003-03-06

  机译： 蔬菜原蛋白的转谷氨酰胺酶交联蛋白的制备方法及其用途

• 5. METHOD FOR PRODUCING TRANSGLUTAMINASE-CROSS-LINKED PROTEINS OF VEGETABLE ORIGIN, PROTEIN GELS AND THE USE THEREOF [P] . 世界知识产权组织专利： WO03017777A1 . 2003-03-06

  机译： 蔬菜原蛋白的转谷氨酰胺酶交联蛋白的制备方法及其用途

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• 1. A new size and shape controlling method for producing calcium alginate beads with immobilized proteins [J] . Yan Zhou ,Shin’ichiro Kajiyama ,Hiroshi Masuhara . 生物医学工程（英文） . 2009,第5期
• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 3. Protein Quality Evaluation of Animal Food Proteins by iIn-Vitro/iMethodologies [J] . Jesús Manuel Barrón-Hoyos ,Adrian Rafael Archuleta ,María del Refugio Falcón-Villa . 食品与营养科学（英文） . 2013,第4期
• 4. A robust protein extraction method for two dimensional electrophoresis of silkworm proteins [J] . Lvgao Qin ,Haifeng Shi ,Hengchuan Xia . 生命科学与技术进展（英文） . 2013,第4期
• 5. A Rapid Electrophoresis Method on Agarose Gel to Characterise Dairy Protein Aggregates [J] . Laetitia Gemelas ,Pascal Degraeve ,Marion Morand . 食品与营养科学（英文） . 2018,第4期
• 6. 血清脂蛋白(a)、纤维蛋白原和超敏C反应蛋白与冠状动脉病变程度关系 [C] . GONG chun ,龚春 ,HUANG shao-lie . 江西省第六次中西医结合心血管学术交流会 . 2013
• 7. 转谷氨酰胺酶交联改善花生分离蛋白凝胶性研究 [A] . 刘砚 . 2010