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COMPETITIVE POPULATION NORMALIZATION FOR COMPARATIVE ANALYSIS OF NUCLEIC ACID SAMPLES
COMPETITIVE POPULATION NORMALIZATION FOR COMPARATIVE ANALYSIS OF NUCLEIC ACID SAMPLES
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机译：核酸样本比较分析中的竞争人口标准化
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摘要
Methods are disclosed that convert two or more complex nucleic acid samples into a single collection of normalized target molecules that can be used to compare the abundance of each of the targets in the original samples. Multiple RNA or DNA samples are uniquely tagged and pooled to create a sample mixture. A defined set of target within the sample mixture is converted to approximately equal amounts of nucleic acid by one of several methods employing primer extension with a set of target specific primers. The concentration of target specific primers is equal and limiting for all targets, therefore an appropriate number of primer extension cycles converts all targets to similar final concentrations of product nucleic acid. The different tags appended to the sample nucleic acids from each sample population unique. These different tags are used to generate RNA or DNA , molecules for analysis that derive form each of the input samples. The disclosed methods are primarily intended to enhance gene array analysis, however, any method used to compare multiple nucleic acid targets form different samples will benefit form the invention.
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