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Method for identifying species of the genus eucalyptus and primer set therefor
Method for identifying species of the genus eucalyptus and primer set therefor
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机译:鉴定桉属的方法及其引物组
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摘要
PROBLEM TO BE SOLVED: To provide a method for identifying tree species in the genus Eucalyptus without influence by autolysis of DNA or microbial DNA by repeatedly applying the PCR-SSCP(polymerase chain reaction-single strand conformation polymorphism) analysis to DNA extracted from a tissue of a tree belonging to the genus Eucalyptus using a plurality of specific primer sets. SOLUTION: This is a method for identifying tree species in the genus Eucalyptus without influence by autolysis of DNA or contamination of microbial DNA by repeatedly applying the PCR-SSCP analysis to DNA extracted from a tissue of a tree belonging to the genus Eucalyptus using two or more specific primer sets comprising two primers different from each other and which are selected from six primers respectively having base sequences represented by formulas I to VI.
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