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Method for identifying species of the genus eucalyptus and primer set therefor

机译:鉴定桉属的方法及其引物组

摘要

PROBLEM TO BE SOLVED: To provide a method for identifying tree species in the genus Eucalyptus without influence by autolysis of DNA or microbial DNA by repeatedly applying the PCR-SSCP(polymerase chain reaction-single strand conformation polymorphism) analysis to DNA extracted from a tissue of a tree belonging to the genus Eucalyptus using a plurality of specific primer sets. SOLUTION: This is a method for identifying tree species in the genus Eucalyptus without influence by autolysis of DNA or contamination of microbial DNA by repeatedly applying the PCR-SSCP analysis to DNA extracted from a tissue of a tree belonging to the genus Eucalyptus using two or more specific primer sets comprising two primers different from each other and which are selected from six primers respectively having base sequences represented by formulas I to VI.
机译:解决的问题:通过对从组织中提取的DNA重复应用PCR-SSCP(聚合酶链反应-单链构象多态性)分析,提供一种鉴定桉树属树种而不受DNA或微生物DNA自溶影响的方法多个特定引物对属于桉属的树木的鉴定。解决方案:这是一种方法,该方法通过使用两个或多个从桉树属树的组织中提取的DNA重复应用PCR-SSCP分析,来鉴定桉树属中的树种,而不受DNA的自溶或微生物DNA的污染。更具体的引物组包含两个互不相同的引物,并且分别选自具有分别由式I至VI表示的碱基序列的六个引物。

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