首页> 外国专利> RECOMBINANT TRIACYLGLYCEROL LIPASE OF LESS THANIGREATER THANARABIDOPSIS THALIANALESS THAN/IGREATER THAN, NUCLEOTIDE SEQUENCES CODING FOR SAME OR CORRESPONDING TO ANTISENSE SEQUENCES AND USES THEREOF

RECOMBINANT TRIACYLGLYCEROL LIPASE OF LESS THANIGREATER THANARABIDOPSIS THALIANALESS THAN/IGREATER THAN, NUCLEOTIDE SEQUENCES CODING FOR SAME OR CORRESPONDING TO ANTISENSE SEQUENCES AND USES THEREOF

机译：重组花生四烯酸甘油三酯脂酶的反义核苷酸序列比反义序列少或多，核苷酸序列编码的反义序列相同或相对应，并使用它们

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Recombinant triacylglycerol (TAG) lipase (I) of Arabidopsis thaliana, and its derivatives and/or variants, are new. Recombinant triacylglycerol (TAG) lipase (I) of Arabidopsis thaliana, and its derivatives and/or variants, are new. (I) has a sequence (2) of 393 amino acids (aa), where the first 21 aa represent a signal peptide, and the mature protein has a 372 aa sequence (4). The variants/fragments may have one or more aa substitutions provided that they retain: (i) at least 70% identity or 80% similarity with (I); and (ii) have the same enzymatic activity as (2), especially ability to hydrolyze oils such as long-chain TAG, specifically triolein. Independent claims are also included for: (1) nucleic acid sequence (II) that encodes (I) or its fragments or variants; (2) recombinant nucleic acid (IIa) that comprises (II) and elements required for transcription, particularly promoter and terminator; (3) vectors e.g. plasmids or baculovirus, that contains (IIa); (4) host cells transformed by the vector of (3); (5) preparation of (I), or its fragments or variants, by culturing cells of (4); (6) antisense nucleic acids (IIb) that are complementary to (II); (7) vector e.g. plasmid or Agrobacterium tumefaciens that contains (IIb); (8) transgenic plant cells, plant parts or plants transformed with (II), (IIa) or (IIb); (9) transgenic seeds transformed with (II), (IIa) or (IIb), produced by crossing plants of (8), and the plants produced by growing them; (10) methods for producing transgenic plants of (8); (11) nutritional supplement or pharmaceutical composition that contains (I), or its fragments or variants; and (12) mono- or poly-clonal antibodies (Ab) directed against (I), or its derivatives and fragments.

机译：拟南芥的重组三酰基甘油（TAG）脂肪酶（I）及其衍生物和/或变体是新的。拟南芥的重组三酰基甘油（TAG）脂肪酶（I）及其衍生物和/或变体是新的。（I）具有393个氨基酸（aa）的序列（2），其中前21个氨基酸代表信号肽，而成熟蛋白具有372个氨基酸序列（4）。变体/片段可以具有一个或多个氨基酸取代，只要它们保留：（i）与（I）至少70％的同一性或80％的相似性; （ii）具有与（2）相同的酶活性，特别是具有水解油的能力，例如长链TAG，特别是三油精。还包括以下方面的独立权利要求：（1）编码（I）或其片段或变体的核酸序列（II）; （2）重组核酸（IIa），其包含（II）和转录所需的元件，特别是启动子和终止子; （3）向量含有（IIa）的质粒或杆状病毒; （4）由（3）的载体转化的宿主细胞; （5）通过培养（4）的细胞来制备（I）或其片段或变体; （6）与（II）互补的反义核酸（IIb）; （7）向量含有（IIb）的质粒或根癌农杆菌; （8）用（II），（IIa）或（IIb）转化的转基因植物细胞，植物部分或植物; （9）通过杂交（8）的植物产生的，经（II），（IIa）或（IIb）转化的转基因种子，以及通过使它们生长而产生的植物; （10）产生（8）的转基因植物的方法; （11）含有（I）或其片段或变体的营养补品或药物组合物; （12）针对（I）或其衍生物和片段的单克隆或多克隆抗体（Ab）。

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公开/公告号AU2003258804A1

专利类型
公开/公告日2003-12-31

原文格式PDF
申请/专利权人 CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE;
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申请/专利号AU20030258804
发明设计人 VINCENT ARONDEL;
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申请日2003-06-13
分类号C12N9/20;A01H5/10;A61K38/46;A61P1/14;C07K16/16;C12P41/00;G01N33/573;A23L1/30;C11D3/386;C11C3/00;C12N15/55;C12N5/10;C12N5/04;C12N15/63;A01H5/00;
国家 AU
入库时间 2022-08-21 23:02:45

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