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Methods for Preparation of Enzymatically Active Lysozyme, Synthetic Gene for Human Lysozyme, and Yeast Cells capable of Expressing the Synthetic Gene
Methods for Preparation of Enzymatically Active Lysozyme, Synthetic Gene for Human Lysozyme, and Yeast Cells capable of Expressing the Synthetic Gene
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机译：制备酶活性溶菌酶，人溶菌酶的合成基因以及能够表达该合成基因的酵母细胞的方法
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摘要
Yeast cells whose DNA has been appropriately engineered to produce and to secrete lysozyme under control of a regulatable promoter are grown in the substantial absence of lysozyme synthesis and then are induced to produce and to secrete lysozyme under growth-limited conditions. This process is particularly suitable for the production of human lysozyme or mutants thereof.
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