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Method of managing the chemotherapy of patients who are HIV positive based on the phenotypic drug sensitivity of human HIV strains and a clinical management device for use in the management of chemotherapy of patients who are HIV positive
Method of managing the chemotherapy of patients who are HIV positive based on the phenotypic drug sensitivity of human HIV strains and a clinical management device for use in the management of chemotherapy of patients who are HIV positive
The proposed method of managing HIV chemotherapy of patients who are HIV positive comprises transfecting a cell line susceptible to infection caused by HIV, with a sequence, preferably that encodes for RT and protease, from the pol gene of HIV obtained from a patient and a HIV-DNA construct from which the sequence has been deleted, culturing the transfected cells so as to create a stock of chimeric viruses, assessing the phenotypic sensitivity of the chimeric viruses to an inhibitor of the enzyme encoded by the pol gene of HIV and assigning a value thereto, constructing a data set comprising the value for chimeric virus sensitivity and the corresponding value for a chimeric wild-type strain of HIV, repeating the sensitivity assessment for at least two further inhibitors and thereby constructing at least three such data sets in total, representing the data sets in two-dimensional or three-dimensional graphical form such that the difference between the chimeric and wild-type virus sensitivities in the case of each data set provides a visual measure of the resistance of the chimeric stock to treatment by the inhibitor in question, and selecting the optimum inhibitor(s) on the basis of the graphical representation of the resistances so measured. Disclosed is also a clinical management device for use in the management of chemotherapy of patients who are HIV positive, said device bearing a graphical representation of a plurality of data sets obtained by the above-described manner.
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