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The sensor amperomu00e9trico for NADH based organic dyes adsorbed on su00eclica gel modified with metal oxides
The sensor amperomu00e9trico for NADH based organic dyes adsorbed on su00eclica gel modified with metal oxides
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机译：吸附在用金属氧化物改性的硅胶上的基于NADH的有机染料的传感器安培数
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摘要
"Amperomu00e9trico sensor for NADH based organic dyes adsorbed on su00eclica gel modified with metal oxides.This case describes the development of highly stable sensors for electrochemical determination of NADH.The developed sensors consist of an electrode of Carbon Paste Modified by the incorporation of the matrix of silica gel coated with metal oxides adsorbed electron mediators DThe type organic dyes, such as fenoxazinas (Meldola blue and Blue Nile) and phenothiazines (toluidine blue).The electrochemical response of the sensor is based on the eletrocatalu00edticas properties of these mediators to oxidize NADH.Illustratively,In this process the responses obtained using the sensor on the basis of the blue meidola adsorbed on silica gel modified with niobium oxide incorporated in carbon paste.The performance of this sensor was investigated and its dependence on the critical factors, such as pH, ionic strength, electrolyte support and working potential.The proposed sensor has excellent stability, sensitivity and wide linear response range, on the potential applied to the V (vs ECS) which entitles the holder to be used, for example,In the construction of biosensors based on enzymes desidrogenases.This sensor was obtained for a range of linear response of 1.0 x 10 ^ - 5 - 7.5 x 10 ^ - 4 ^ mol L ^ - 1 ^ of NAD ^ + ^, with sensitivities in the order of 48 Ma l mol ^ - 1 ^.The response time to reach a maximum of signal was 1 s. The repeatability, expressed as relative standard deviation, was 3% for n = 10.This sensor was very stable, being able to carry out more than 300 analyses without significant changes.
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机译：用于吸附在金属氧化物改性的硅胶上的基于NADH的有机染料的安培传感器。本案例描述了用于电化学测定NADH的高度稳定的传感器的开发。开发的传感器由碳糊修饰的碳糊电极组成。结合了涂覆有金属氧化物的硅胶基质吸附了电子介体D型有机染料，如fenoxazinas（Meldola蓝和Blue Nile）和吩噻嗪（甲苯胺蓝）。传感器的电化学响应基于电催化的性质说明性地，在该过程中，该传感器基于吸附在掺有碳糊的氧化铌改性的硅胶上的蓝色中膜上获得的响应。研究了该传感器的性能及其对电极的依赖性。 pH，离子强度，电解质支持和工作电势等关键因素。在V（vs ECS）上施加的电位上具有稳定的稳定性，灵敏度和宽线性响应范围，该电位使持有人有权使用，例如在基于脱氢酶的生物传感器的构建中。线性响应为1.0 x 10 ^-5-7.5 x 10 ^-4 ^ mol L ^-1 ^ NAD ^ + ^，灵敏度为48 Ma l mol ^-1 ^，响应时间达到a信号最大值为1 s。对于n = 10，以相对标准偏差表示的可重复性为3％。此传感器非常稳定，能够执行300多次分析而无明显变化。
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