• 主题
高级搜索  >
历史搜索 清空历史
首页> 外国专利> Process for purification of soluble proteins from the bristles of l. oblique with prothrombin activating activity; process for partially determining the prothrombin activator amino acid sequence; process of determining prothrombin activating activity of fraction ii, n-terminal sequence and sequence of internal fragments of prothrombin activating fraction, prothrombin activator and use of prothrombin activator

Process for purification of soluble proteins from the bristles of l. oblique with prothrombin activating activity; process for partially determining the prothrombin activator amino acid sequence; process of determining prothrombin activating activity of fraction ii, n-terminal sequence and sequence of internal fragments of prothrombin activating fraction, prothrombin activator and use of prothrombin activator

机译：从1的刚毛中纯化可溶性蛋白的方法。斜向具有凝血酶原激活活性;部分确定凝血酶原活化剂氨基酸序列的方法; Ⅱ的凝血酶原活化活性的过程，凝血酶原活化级分的n-末端序列和内部片段的序列，凝血酶原活化剂以及凝血酶原活化剂的用途

页面导航
  • 摘要
  • 著录项
  • 相似文献

摘要

"PROCESS OF SOLUBLE PROTEINS OF L. OBLIQUA CERES WITH PROTROMBIN ACTIVATIVE ACTIVITY; PROCESS FOR PARTIAL DETERMINATION OF PROTROMBIN ACTIVATOR SEQUENCES; OF INTERNAL FRAGMENTS OF PROTROMBIN ACTIVATING FRACTION, PROTROMBIN ACTIVATOR AND USE OF PROTROMBIN ACTIVATOR ". The present invention relates to a process for purifying soluble proteins of L. obliqua bristles with prothrombin activating activity; the process for partial determination of the prothrombin activator amino acid sequence; The process for determining the prothrombin activating activity of fraction II as well as the n-terminal sequence and sequence of internal fragments of the prothrombin activating fraction to the prothrombin activator and the use of the prothrombin activator from the homogenization of the L. bristles. oblique. The present invention proves that a single component of the Lonomia obliqua venom, Lopap, causes hemorrhagic syndrome directly by the activation of prothrombin and therefore should refer therapy in the event of accidents with Lonomia obliqua. According to the present invention Lopap is a new prothrombin activator, which is an important factor responsible for the consumption coagulopathy found in L. obliqua poisoned patients. At low doses the purified protein, by its ability to activate prothrombin generating thrombin, withdraws fibrinogen from the circulation in a controlled manner, transforming it into fibrin microcoagles. This decrease in plasma fibrinogen concentration allows the blood clotting time to extend and therefore prevents acute vascular thrombosis. Because it lacks proteolytic activity, the protein would maintain the coagulant capacity of fibrinogen not consumed in the process. Thus the plasma concentration of fibrinogen would be decreased, but there would be no predisposition to a hemorrhagic state. In addition, it could be used in the manufacture of diagnostic KITS for detection of plasma prothrombin.
机译：“具有原蛋白原活性的可溶乳杆菌蛋白质的过程;部分测定原蛋白原激活物的过程;原蛋白原内在片段中的活性部分，原蛋白原激活物和使用原蛋白。”本发明涉及一种具有凝血酶原激活活性的纯化斜生猪鬃毛可溶性蛋白的方法。部分确定凝血酶原活化剂氨基酸序列的方法;用于确定级分II的凝血酶原激活活性以及凝血酶原激活级分对凝血酶原激活剂的n-末端序列和内部片段的序列的方法，以及使用来自刚毛乳杆菌均质化的凝血酶原激活剂的用途。斜。本发明证明了Lonomia obliqua蛇毒的单个成分Lopap直接通过凝血酶原的激活直接引起出血综合征，因此在发生Lonomia obliqua事故时应参考治疗。根据本发明，Lopap是一种新的凝血酶原激活剂，它是导致在L.obliqua中毒患者中发现消耗性凝血病的重要因素。在低剂量下，纯化的蛋白质通过其激活凝血酶原产生凝血酶的能力，以受控方式从循环中吸收纤维蛋白原，将其转化为纤维蛋白微凝集素。血浆纤维蛋白原浓度的这种降低使血液凝结时间延长，从而防止了急性血管血栓形成。由于它缺乏蛋白水解活性，因此该蛋白质将保持该过程中未消耗的纤维蛋白原的凝结能力。因此，血纤蛋白原的血浆浓度将降低，但是不会有出血状态的倾向。此外，它还可用于诊断KITS的制造，以检测血浆凝血酶原。

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号