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Method of exploration of the genome, based on map for identification of lakes regulators which control the degree of transcripts and gene products

机译:基于地图的基因组探索方法,用于识别控制转录物和基因产物程度的湖泊调节剂

摘要

"Method of exploration of the genome, based on map for identification of lakes regulators which control the degree of transcripts and gene products.The invention relates to a method for identifying one or more regions within a genome of an organism of interest, which is the mediation of expression of one or more genes of interest.The method comprises identifying a first and a second organism of interest.The first body of interest being characterized by exhibiting a measurable response to an environmental stimulus or by exhibiting a phenotype associated with the expression of the gene differential AssocIada with a process of interest.The second organism of interest is characterized by omitting or does not display a strong response to the stimulus.As observed in the first organism of interest or display a different phenotype compared with the first body of interest.In the different phenotype is associated with the process of interest.Or display a phenotype of interest that is secreted when compared with the first body of interest or a combination of these.The first and second bodies of interest are crossed to produce a population of progeny segregated and the RNA is extracted from each progeny segregated.The level of expression of the gene of one or more genes of interest that are associated with the response to environmental stimuli or the process of interest is quantified.It is prepared, a linkage map of progeny segregated.Using one or more markers and the relationship between said one or more markers on the linkage map and the gene expression of said one or more genes of interest is determined and one or more lakes characteru00edstica quantitative (QTL) identified.This method also relates to the identification of one or more QTLs associated with one or more genes of interest in the segregated progeny, which is subjected to a desired environmental stimulus.In addition,This method can be used to identify one or more QTLs corresponding to a transcription factor or any factor that controls the expression of one or more genes of interest and saidOne or more genes located in said one or more QTLs can be isolated and characterized.
机译:基于图谱的基因组探索方法,用于鉴定控制转录物和基因产物程度的湖泊调节剂。本发明涉及一种鉴定感兴趣生物体基因组中一个或多个区域的方法。该方法包括鉴定第一个和第二个目标生物。第一个目标生物的特征是对环境刺激表现出可测量的反应或表现出与表达相关的表型第二个目标生物的特征是忽略或不表现出强烈的刺激反应。在第一个目标生物中观察到,或与第一个目标生物相比表现出不同的表型在不同的表型中与感兴趣的过程相关联。或显示一个由W分泌的感兴趣的表型与第一个或第二个目标抗体相比,第一个和第二个目标抗体杂交产生分离的子代群体,并从每个分离的子代中提取RNA。一个或一个基因的表达水平量化与环境刺激反应或感兴趣过程相关的更多感兴趣基因。制备,分离后代的连锁图。使用一个或多个标记以及连锁图上所述一个或多个标记之间的关系并确定所述一种或多种目的基因的基因表达,并鉴定一种或多种湖泊性状(QTL)。该方法还涉及鉴定与所述一种或多种目的基因相关的一种或多种QTL。分离的后代,可承受所需的环境刺激。此外,此方法可用于识别一个或多个与转录因子或转录因子相对应的QTL。控制一个或多个目的基因和所述表达的ny因子可以被分离和表征位于所述一个或多个QTL中的一个或多个基因。

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