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Method for the detection of minority genomes in quasi species and kit for detection of viral genomes in minority in quasi species viral

机译：准物种中少数基因组的检测方法和准病毒中少数基因组的检测试剂盒

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Method for the detection of minority genomes in quasi species and kit for detection of viral genomes in minority in almost - viral species.Which method allows the detection of minority genomes, in particular, minority genomes memory, of a population of nucleic acids to a quasi species viral.Present in a proportion of less than 50%, containing at least a mutation in the majority of the quasi species genome.And comprises: (a) extracting the nucleic acid of the almost - viral species from a sample suspected to contain the viral quasi species; (b) to amplify at least.A fragment of the nucleic acid of the quasi species, viral; and (c) to identify and analyse the existence of minority genomes, using techniques based on the use of DNA microchips.The test of the printing heteroduplex (HTA) and molecular cloning. The method has application in the genetic diagnosis of viral diseases.
机译：准物种中少数基因组的检测方法和近病毒物种中少数物种的病毒基因组的检测试剂盒。该方法允许检测准核酸群体的少数基因组，特别是少数基因组的记忆以少于50％的比例存在，在大多数准物种基因组中至少包含一个突变，并且包括：（a）从怀疑含有该病毒的样品中提取几乎是病毒的核酸。病毒准种; （b）至少扩增半病毒的核酸片段; （c）使用基于DNA微芯片的技术鉴定和分析少数基因组的存在。印刷异源双链（HTA）和分子克隆的测试。该方法已应用于病毒性疾病的遗传诊断。

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