首页> 外国专利> Isolated polynucleotide, First and second polynucleotide primer, method to detect the occurrence of Cotton Plant 531, isolated polynucleotide Molecule obtained by Said Method, Nucleic Acid detection equipment and Method for determining the cigosidad Genome of a Cotton Plant.

Isolated polynucleotide, First and second polynucleotide primer, method to detect the occurrence of Cotton Plant 531, isolated polynucleotide Molecule obtained by Said Method, Nucleic Acid detection equipment and Method for determining the cigosidad Genome of a Cotton Plant.

机译:分离的多核苷酸,第一和第二多核苷酸引物,检测棉花植物531的发生的方法,通过所述方法获得的分离的多核苷酸分子,核酸检测设备和用于确定棉花植物的蝉毛基因组的方法。

摘要

Compositions and Methods for Detecting the presence of several genomic regions / insertion of a transgenic Cotton Plant called PV ghbk04, also known as this documentation in the event of Cotton 531.There are DNA sequences that comprise at least one sequence of genomic region of 531 transgenic insertion / sec id identified as SEQ ID No.1, No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID no32 and Accessories thereof,Where a sequence of insertion insertion region encompasses the Union between the heterologous DNA inserted into the Genome of Cotton and Cotton Genome DNA surrounding the insertion site and is Diagnostic of the event.The DNA sequences that comprise at least 11 or more contiguous Nucleotide sequence of SEQ ID no. 1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID no32, SEQ ID no. 12, SEQ ID no. 13, SEQ ID no 17, SEQ ID no 18, SEQ ID no. 19, SEQ ID no 20, SEQ ID SEQ ID no. 21, 22, 23 SEQ ID no, SEQ ID NO.24, SEQ ID no. 25, SEQ ID no. 26, SEQ ID no. 27,SEQ ID no 28 and Accessories of the same for use as primers for DNA Amplification methods and DNA amplicons produced using these primers that are Diagnostic for the occurrence of Cotton.Methods to detect the presence of DNA corresponding to the event in a sample of 531, Nucleic Acid detection equipment, and the method to determine the cigosidad Genome of Cotton Plant was also described.
机译:用于检测转基因棉花植物PV ghbk04的几个基因组区域/插入的成分和方法,在棉花531事件中也称为本文档.DNA序列至少包含一个基因组区域531的序列标识为SEQ ID No.1,No.2,SEQ ID No.3,SEQ ID No.4,SEQ ID No32及其附件的转基因插入/秒ID,其中插入插入区的序列包含异源之间的联合插入棉花基因组的DNA和插入位点周围的棉花基因组DNA,可对该事件进行诊断。该DNA序列包含至少11个或更多连续的SEQ ID No. 1,SEQ ID No.2,SEQ ID No.3,SEQ ID No.4,SEQ ID no32,SEQ ID no。 SEQ ID NO.12, 13,SEQ ID NO 17,SEQ ID NO 18,SEQ ID NO。 SEQ ID No.19,SEQ ID No 20。 SEQ ID NO.21、22、23,SEQ ID NO.24,SEQ ID NO。 25,SEQ ID no。 SEQ ID No.26。 27,SEQ ID no 28及其附件,用作DNA扩增方法的引物,以及使用这些引物产生的DNA扩增子,可对棉花的发生进行诊断。检测样品中与事件对应的DNA的存在的方法还描述了核酸检测设备531,以及测定棉花植物中洋蓟花基因组的方法。

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