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COMPETITIVE HYBRIDIZATION METHOD IN GENE EXPRESSION ANALYSIS BY USING DNA MICROARRAY AND CONTROL RNA USED FOR GENE EXPRESSION ANALYSIS BY DNA MICROARRAY
COMPETITIVE HYBRIDIZATION METHOD IN GENE EXPRESSION ANALYSIS BY USING DNA MICROARRAY AND CONTROL RNA USED FOR GENE EXPRESSION ANALYSIS BY DNA MICROARRAY
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机译：DNA微阵列的竞争性杂交方法在基因表达分析中的应用和控制RNA用于DNA微阵列的基因表达分析
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摘要
PROBLEM TO BE SOLVED: To measure effective data in a gene, etc., incapable of accurately detecting a ratio of the expression amount of a sample RNA to the expression amount of the control RNA by existing measuring techniques of DNA microarray because these expression amounts are remarkably different between the sample RNA and the control RNA.;SOLUTION: The sample RNA extracted and amplified from a biopsy specimen such as a human liver tissue is labeled with Cy3 by using a reverse transcriptase to prepare a sample. A spike RNA (an RNA existing in both of the sample RNA and the control RNA in which the signal strength of the sample is higher than the signal strength of the control or an RNA, existing in the sample RNA, in which the existence is not recognized in the control RNA) is added to the control RNA extracted and amplified from a human cultured cell, etc. and the mixture is labeled with Cy5 by using the reverse transcriptase to prepare the sample.;COPYRIGHT: (C)2004,JPO
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