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Real-time monitoring of protein activation, useful in biosensors for identifying inducers of stress or chemotaxis, using transient fluorescence from modified proteins

机译：实时监测蛋白质活化，可用于生物传感器中，用于识别应激或趋化性诱导物，它利用修饰蛋白质的瞬时荧光

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摘要

Genetic engineering method for real-time monitoring, in a genetically modified microorganism, of the activation of specific proteins (I), involved in transduction of cellular signals, that can bind phosphate and/or can be phosphorylated on amino acid residues. Genetic engineering method for real-time monitoring, in a genetically modified microorganism, of the activation of specific proteins (I), involved in transduction of cellular signals, that can bind phosphate and/or can be phosphorylated on amino acid residues. The method comprises integrating, into (I), fluorescent molecular probes (II), shifted in wavelength and detectable by measuring transient fluorescence with almost immediate return of the microorganism to base level after abolition or disappearance of a constraint (environmental stress or chemotaxis) imposed on the microorganism.

机译：用于在转基因微生物中实时监测涉及细胞信号转导的特定蛋白质（I）激活的基因工程方法，该信号可以结合磷酸盐和/或可以在氨基酸残基上被磷酸化。用于在转基因微生物中实时监测涉及细胞信号转导的特定蛋白质（I）激活的基因工程方法，该信号可以结合磷酸盐和/或可以在氨基酸残基上被磷酸化。该方法包括将波长变化的荧光分子探针（II）整合到（I）中，并且可以通过测量瞬时荧光来检测，该瞬时荧光几乎消除了施加的限制（环境压力或趋化性）后微生物几乎立即返回到基础水平。在微生物上。

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公开/公告号FR2828692A1

专利类型
公开/公告日2003-02-21

原文格式PDF
申请/专利权人 DELEGATION GENERALE POUR LARMEMENT DIRECTION DES SYSTEMES DE FORCES ET DE LA PROSPECTIVE (SREA);
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申请/专利号FR20010010840
发明设计人 SIMON PHILIPPE;
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申请日2001-08-16
分类号C12Q1/02;C12Q1/42;C12Q1/68;
国家 FR
入库时间 2022-08-21 23:37:56

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