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Preparing an antiangiogenic form of antithrombin (AT) III, useful for treating tumors and leukemia, comprises incubating native human ATIII with tumor cells, performing an endothelial cell proliferation assay, analysis, and purification
Preparing an antiangiogenic form of antithrombin (AT) III, useful for treating tumors and leukemia, comprises incubating native human ATIII with tumor cells, performing an endothelial cell proliferation assay, analysis, and purification
Preparing antiangiogenically active antithrombin III (ATIII), comprising: (i) incubating native human ATIII with tumor cells; (ii) performing a basic fibroblast growth factor (bFGF) stimulated endothelial cell proliferation assay; (iii) analyzing the modified ATIII by Western blotting and urea-gradient gel analysis; and (iv) purification on heparin-Sepharose and anion-exchange columns, is new. Preparing antiangiogenically active antithrombin III (ATIII) comprising: (i) culturing a tumor cell line to confluence; (ii) incubating in serum-free medium for 48 hours; (iii) adding 1 mg/mL of native human ATIII; (iv) incubating for 48 hours; (v) recovering the culture supernatant; (vi) performing a bFGF (basic fibroblast growth factor)-stimulated endothelial cell proliferation assay; (vii) analyzing the modified ATIII by Western blotting and urea-gradient gel analysis; and (viii) purification on heparin-Sepharose and anion-exchange columns, is new. Independent claims are also included for: (1) use of coagulation-active (native) ATIII for treating and preventing diseases, especially those associated with angiogenesis; and (2) antiangiogenically active ATIII prepared by the new method.
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