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Method of building up a library of substances, comprises separating mixtures using high performance liquid chromatography, and comparing retention time peaks under standard conditions versus laboratory conditions

机译:建立物质库的方法,包括使用高效液相色谱法分离混合物,以及比较标准条件与实验室条件下的保留时间峰

摘要

Method of discovering mixtures of substances to build up a substance library, comprises: (A) separating mixtures by chromatographic or electrophoretic means; and (B) identifying low molecule organic substances in each mixture by the physical parameters of retention time and the molecular mass registered by mass spectrometry. Method of discovering mixtures of substances to build up a substance library, comprises: (a) separating mixtures by chromatographic or electrophoretic means; and (b) identifying low molecule organic substances in each mixture by the physical parameters of retention time and the molecular mass registered by mass spectrometry. The high performance liquid chromatograph (HPLC) diagram (10) of a standard solution under standard conditions gives two peaks (12,14) for retention times of reference measurements. In a subsequent diagram (16) under laboratory conditions, the corresponding peaks (18,20) of the same substances are shifted (22,24) against the previous peaks.
机译:发现物质混合物以建立物质库的方法,包括:(A)通过色谱或电泳方法分离混合物; (B)通过保留时间的物理参数和通过质谱法记录的分子量来鉴定每种混合物中的低分子有机物。发现物质混合物以建立物质库的方法,包括:(a)通过色谱或电泳手段分离混合物; (b)通过保留时间的物理参数和通过质谱法记录的分子量来鉴定每种混合物中的低分子有机物。标准溶液在标准条件下的高效液相色谱(HPLC)图(10)给出了两个峰(12,14),用于参考测量的保留时间。在实验室条件下的下一个图表(16)中,相同物质的相应峰(18,20)与先前的峰相对移(22,24)。

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