首页> 外国专利> SMALL SUBUNIT OF ISOZYME III AND ISOZYME III OF ACETOHYDROXYACID SYNTHETASE FROM ESCHERICHIA COLI, DNA FRAGMENT (VARIANTS), STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF L-VALINE (VARIANTS) AND METHOD FOR PREPARING L-VALINE

SMALL SUBUNIT OF ISOZYME III AND ISOZYME III OF ACETOHYDROXYACID SYNTHETASE FROM ESCHERICHIA COLI, DNA FRAGMENT (VARIANTS), STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF L-VALINE (VARIANTS) AND METHOD FOR PREPARING L-VALINE

机译：大肠埃希氏菌，DNA片段（变体），产L-缬氨酸（变体）的产大肠埃希氏菌的菌株的乙醛酸合成酶的同工酶III和同工酶III的小亚基和制备L-缬氨酸的方法

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FIELD: molecular biology, biochemistry, amino acids, microbiology. SUBSTANCE: invention relates to method for preparing L-valine. The strain of E. coli comprising DNA encoding isozyme III of acetohydroxyacid synthetase from Escherichia coli is cultured in nutrient medium providing production and accumulation of L-valine in nutrient medium and isolation of L-valine from nutrient medium. Activity of isozyme III of synthetase is not inhibited by L-valine and exhibits catalytic activity in two reactions: reaction of synthesis of α-acetolactate from pyruvate and reaction of synthesis of α-aceto-α-hydroxybutyrate from α-ketobutyrate and pyruvate. Isozyme III in small subunit comprises mutation in position 17 with substitution of serine for phenylalanine or in position 29 with substitution of asparagine residue for lysine or tyrosine, or mutation resulting to deletion of C-terminal site beginning from amino acid in position 91 or to substitution of glycine residue in position 14 for aspartic acid residue. Amino acid sequences are given in the description. Strains of E. coli comprise corresponding DNA fragments. EFFECT: enhanced expression of L- valine. 10 cl, 2 dwg, 4 tbl

机译：领域：分子生物学，生物化学，氨基酸，微生物学。发明领域本发明涉及制备L-缬氨酸的方法。在营养培养基中培养包含编码来自大肠杆菌的乙酰羟酸合成酶的同工酶III的DNA的大肠杆菌菌株，以在营养培养基中产生和积累L-缬氨酸，并从营养培养基中分离出L-缬氨酸。合成酶的同工酶III的活性不受L-缬氨酸的抑制，并在两个反应中表现出催化活性：由丙酮酸合成α-乙酰乳酸的反应和由α-酮丁酸酯合成α-乙酰-α-羟基丁酸酯的反应。和丙酮酸。小亚基中的同工酶III包含第17位突变为丝氨酸取代苯丙氨酸，或第29位突变为天冬酰胺残基取代赖氨酸或酪氨酸，或突变导致从91位氨基酸开始的C末端位点缺失或取代14位甘氨酸残基的天冬氨酸残基。氨基酸序列在说明书中给出。大肠杆菌菌株包含相应的DNA片段。效果：L-缬氨酸表达增强。 10厘升，2载重吨，4桶

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公开/公告号RU2209246C2

专利类型
公开/公告日2003-07-27

原文格式PDF
申请/专利权人 ZAKRYTOE AKTSIONERNOE OBSHCHESTVO NAUCHNO-ISSLEDOVATELSKIJ INSTITUT ADZHINOMOTO-GENETIKA;
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申请/专利号RU20000101678
发明设计人 GORSHKOVA N.V.;LIVSHITS V.A.;AKHVERDJAN V.Z.;GUSJATINER M.M.;BELAREVA A.V.;IVANOVSKAJA L.V.;KHURGES E.M.;DOROSHENKO V.G.;KOZLOV JU.I.;
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申请日2000-01-26
分类号C12N15/31;C12N15/52;C12N15/70;C12P13/08;C12N1/21;C12N1/21;C12R1/19;
国家 RU
入库时间 2022-08-21 23:43:40

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