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The colorimetric quantification of microcystin using protein phosphatase
The colorimetric quantification of microcystin using protein phosphatase
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机译：使用蛋白质磷酸酶比色法测定微囊藻毒素
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摘要
PURPOSE: A colorimetric quantification of microcystin using protein phosphatase is provided, thereby the amount of inorganic phosphrous can be simply and cheaply measured. CONSTITUTION: The colorimetric quantification of microcystin comprises the steps of: adding protein phosphatase and phosphorylase a as a template into a microcystin-containing sample; analyzing the amount of inorganic phosphrous(PO43-) released from the phosphorylase a, in which quantitative analysis of the amount of inorganic phosphrous is carried out by determining the optical density using a coupler, wherein the coupler contains ammonium molybdate, sulfuric acid, potassium antimonyl tartrate and ascorbic acid; and a microplate reader is used for the quantitative analysis of microcystin.
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