METHOD OF ADMINISTERING FimH PROTEIN AS A VACCINE FOR URINARY TRACT INFECTIONS

• 1. 改良的基于热休克蛋白的疫苗和免疫治疗 [P] . 中国专利： CN1816348A . 2006-08-09
• 2. 一种改良型的冻干甲型肝炎减毒活疫苗稳定剂、疫苗半成品、疫苗成品及其制备方法 [P] . 中国专利： CN110585439A . 2019-12-20
• 3. METHOD OF ADMINISTERING FimH PROTEIN AS A VACCINE FOR URINARY TRACT INFECTIONS [P] . 欧洲知识产权局专利： EP1313502A4 . 2005-10-19

  机译： 改良FimH蛋白作为尿道感染疫苗的方法

• 4. Method of administering FimH protein as a vaccine for urinary tract infections [P] . 美国专利： US2005196408A1 . 2005-09-08

  机译： 施用FimH蛋白作为尿路感染疫苗的方法

• 5. Method of administering FimH protein as a vaccine for urinary tract infections [P] . 美国专利： US2003138449A1 . 2003-07-24

  机译： 施用FimH蛋白作为尿路感染疫苗的方法

• 1. Outpatient Urinary-Tract-Infection-Like Symptoms: Causative Microbial Survey Utilizing Multiplex Quantitative Polymerase Chain Reaction Methodology [J] . Vijay Singh, Carrie Wilks, Jairus Reddy, 传染病进展（英文） . 2020,第001期
• 2. Urinary Myeloperoxidase to Creatinine Ratio as a New Marker for Diagnosis of Urinary Tract Infection [J] . Mingjian Bai, Jing Feng, Guowei Liang 中国医学科学杂志（英文版） . 2018,第003期
• 3. Efficacy of antimicrobial impregnated urinary catheter in thwarting foreign body associated urinary tract infections caused by Pseudomonas aeruginosa [C] . Hina Saini, Sanjay Chhibber, Kusum Harjai World biomaterials congress . 2016
• 4. The yersiniabactin system is a uropathogenic Escherichia coli virulence factor and novel vaccine target to prevent urinary tract infection. [D] . Brumbaugh, Ariel Ray. 2014
• 5. Optimization and qualification of an assay that demonstrates that a FimH vaccine induces functional antibody responses in women with histories of urinary tract infections [O] . Courtney M. Starks, Michelle M. Miller, Peter M. Broglie, 2021
• 6. Construction and evaluation of vaccine candidate fimH/fliC against Escherichia coli urinary tract infection [O] . Karam M. Asadi, Oloomi M., Habibi M., 2012
• 7. Evaluation of a Self-Administered Intravaginal Swab for PCR Detection of Genitourinary Tract Infections Including Chlamydia, Gonorrhea, Trichomonas and Human Papillomavirus in Active Duty Military Women. [R] . Rompalo, A. M. 1998

• 1. New possibilities in treatment of chronic urinary tract infections-vaccines, autovaccines and beta glucans [J] . Josef Richter ,Vaclav Vetvicka ,Karola Haasova . 临床与病理杂志 . 2021,第009期
• 2. Bacterial Urinary Tract Infections among Males with Lower Urinary Tract Obstruction at Komfo Anokye Teaching Hos-pital, Kumasi, Ghana [J] . Christian Kofi Gyasi-Sarpong ,Edwin Mwintiereh Ta-ang Yenli ,Ali Idriss . 泌尿学期刊（英文） . 2012,第3期
• 3. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 4. 利用生物交联技术制备细菌多糖-蛋白结合疫苗的方法 [C] . 孙鹏 ,潘超 ,刘波 . 中国生物工程学会2014年学术年会暨全国生物技术大会 . 2014
• 5. 耐低磷苎麻筛选及多年生植物成熟叶片蛋白提取方法改良 [A] . 钟新月 . 2016