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A method of producing a taxane-type diterpene and method of obtaining cultured cells which produce the taxane-type diterpene at a high rate

机译：紫杉烷型二萜的生产方法和获得以高速率生产紫杉烷型二萜的培养细胞的方法

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摘要

This invention relates to a method of producing a taxane-type diterpene(s) wherein tissues or cells of a plant which produces taxane-type diterpene(s) is cultured in the presence of at least one selected from the group consisting of jasmonic acids, compounds containing a heavy metal, complex ions containing a heavy metal, heavy metal ions, amines and antiethylene agents, a method of producing a taxane-type diterpene wherein the tissues or the cells of the plant are cultured by controlling the oxygen concentration in a gas phase in a culture vessel to less than the oxygen concentration in the atmosphere from the initial stage of the culture, or by controlling the dissolved oxygen concentration in a fluid medium which is in contact with the tissue or the cell to less than the saturated dissolved oxygen concentration at that temperature from the initial stage of the culture, a method of producing a taxane-type diterpene wherein the tissue or the cell of the plant is cultured in a culture vessel, while oxygenic gas containing 0.03 - 10 % of carbon dioxide is used as aeration gas to be introduced to the vessel, and a method of obtaining highly productive cultured cells for the taxane-type diterpene wherein cultured cells of the plant which produces the taxane-type diterpene are separated into a plurality of layers according to the difference in their specific gravities, and the cells contained in at least one layer are cultured, then highly productive cultured cells for the taxane-type diterpene are selected from among those cultured cells. The present invention allows industrial production of a taxane-type diterpene such as taxol which is useful as a therapeutic agent for cancer.

机译：本发明涉及一种生产紫杉烷型二萜的方法，其中在至少一种选自茉莉酸的存在下，培养产生紫杉烷型二萜的植物的组织或细胞，含有重金属的化合物，含有重金属的复合离子，重金属离子，胺和抗乙烯剂，一种紫杉烷型二萜的生产方法，其中通过控制气体中的氧气浓度来培养植物的组织或细胞从培养的初始阶段起，将培养皿中的氧气浓度降低到大气中的氧气浓度以下，或者将与组织或细胞接触的流体介质中的溶解氧浓度控制为低于饱和溶解氧浓度从培养的初始阶段开始在该温度下浓缩，一种生产紫杉烷型二萜的方法，其中在培养物中培养植物的组织或细胞一种含有0.03-10％二氧化碳的氧气作为曝气气体引入容器的方法，以及一种用于生产紫杉烷型二萜的高产培养细胞的方法，其中生产紫杉烷的植物的培养细胞根据它们的比重的差异将β-型二萜分离为多个层，并培养至少一层中包含的细胞，然后从这些培养的细胞中选择紫杉烷型二萜的高产培养细胞。本发明允许工业生产紫杉烷型二萜，例如紫杉醇，其可用作癌症的治疗剂。

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公开/公告号EP1063300B1

专利类型
公开/公告日2003-09-03

原文格式PDF
申请/专利权人 MITSUI CHEMICALS INC.;
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申请/专利号EP20000115406
发明设计人 YUKIMUNE YUKIHITOC/O MITSUI CHEMICALS INC.;HARA YOSUHIROC/O MITSUI CHEMICALS INC.;HIGASHI YOSUKEC/O MITSUI CHEMICALS INC.;OHNISHI NAOTOC/O MITSUI CHEMICALS INC.;TABATA HOMAREC/O MITSUI CHEMICALS INC.;SUGA CHUZOC/O MITSUI CHEMICALS INC.;MATSUBARA KOUICHIMITSUI CHEMICALS INC.;
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申请日1994-11-09
分类号C12P17/02;C12N5/04;
国家 EP
入库时间 2022-08-21 23:52:38

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