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METHOD FOR PURIFYING PLASMID DNA AND PLASMID DNA SUBSTANTIALLY FREE OF GENOMIC DNA
METHOD FOR PURIFYING PLASMID DNA AND PLASMID DNA SUBSTANTIALLY FREE OF GENOMIC DNA
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机译:纯化质粒DNA和质粒DNA基本不含基因组DNA的方法
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摘要
A method is described for purifying plasmid DNA from a mixture containing plasmid DNA and genomic DNA. A solution containing both plasmid DNA and genomic DNA is treated with at least 80 % by weight saturation ammonium sulfate, thereby precipitating the genomic DNA and providing purified plasmid DNA in solution. Genomic DNA levels in the purified plasmid DNA product are less than 1 % by weight based on the plasmid DNA. The purified plasmid DNA is suitable for use in humans.
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