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Semi-synthetic antibody fragment combinatorial library based on avian immunoglobulin genes

机译:基于禽免疫球蛋白基因的半合成抗体片段组合文库

摘要

According to the invention there is provided a semi-synthetic antibody fragment combinatorial library based on avian immunoglobulin genes. The avian immunoglobulin genes are poultry or chicken immunoglobulin genes. The antibodies are single chain antibody fragments and the library is displayed on large phages. At least some of the third complementarity determining region (CDR 3) of the variable (V) domain of the heavy (H) chains of the antibody fragments are replaced by synthetically randomised peptides. Synthetically randomised variable heavy (VH) regions are paired with the natural repertoire of avian VH regions and not with defined light chains, the arrangement being such that combinatorial diversity is contributed by both the variable light (VL) and VH regions and further such that the natural VH repertoire of the avian VH regions adds further diversity.
机译:根据本发明,提供了基于禽免疫球蛋白基因的半合成抗体片段组合文库。禽免疫球蛋白基因是家禽或鸡的免疫球蛋白基因。抗体是单链抗体片段,文库显示在大噬菌体上。抗体片段的重(H)链的可变(V)结构域的第三互补决定区(CDR 3)中的至少一些被合成随机化的肽替代。合成随机可变重链(VH)区与禽类VH区的自然组成部分配对,但未与定义的轻链配对,这种排列方式使得可变轻链(VL)和VH区两者都具有组合多样性,而且鸟类VH区的自然VH种类增加了进一步的多样性。

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