首页> 外国专利> STABLE ISOTOPE-LABELED AMINO ACID AND METHOD FOR INCORPORATING THE SAME INTO TARGET PROTEIN, NMR METHOD FOR THE STRUCTURAL ANALYSIS OF PROTEIN AND METHOD FOR PRODUCING REGIO-SELECTIVE STABLE ISOTOPE-LABELED FUMARIC ACID AND TARTARIC ACID

STABLE ISOTOPE-LABELED AMINO ACID AND METHOD FOR INCORPORATING THE SAME INTO TARGET PROTEIN, NMR METHOD FOR THE STRUCTURAL ANALYSIS OF PROTEIN AND METHOD FOR PRODUCING REGIO-SELECTIVE STABLE ISOTOPE-LABELED FUMARIC ACID AND TARTARIC ACID

机译：稳定同位素标记的氨基酸及其将其掺入目标蛋白的方法，用于结构化蛋白质分析的NMR方法以及制备区域选择性稳定的同位素标记的富马酸和酒石酸的方法

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摘要

It is intended to provide a stable isotope-labeled amino acid which is at least one of amino acids constituting protein, characterized by having at least one of the following labeling patterns: (a) in one or more methylene groups, methylene hydrogen atoms other than at least one have been deuterate d; (b) in prochiral gem-methyl groups, hydrogen atoms in one of the methyl grou ps have been completely deuterated; (c) in prochiral gem-methyl groups, hydroge n atoms in the methyl groups have been partially deuterated; and (d) hydrogen atoms in a methyl group other than one have been deuterated while hydrogen atoms in an aromatic ring have been partially deuterated. Using this stable isotope-labeled amino acid, deuterium substitution of a protein can be achieved without worsening the NMR sensitivity of the residual hydrogen nucleus. Thus, an NMR spectrum of a high-molecular weight protein exceeding the upper limit applicable in the prior art can be quickly and surely analyz ed and the stereostructure can be highly precisely determined at the same time.

机译：本发明旨在提供一种稳定的同位素标记的氨基酸，该氨基酸是构成蛋白质的氨基酸中的至少一种，其特征在于具有至少一种以下标记模式：（a）在一个或多个亚甲基中，除d）至少有一位（b）在手性宝石甲基中，一个甲基组中的氢原子已完全氘化; （c）在前手性的甲基中，甲基中的氢原子部分氘化; （d）除了一个以外的甲基中的氢原子被氘化而在芳香环中的氢原子被部分氘化。使用这种稳定的同位素标记的氨基酸，可以实现蛋白质的氘取代，而不会损害残留氢核的NMR敏感性。因此，可以快速且可靠地分析超过现有技术中可应用的上限的高分子量蛋白质的NMR光谱，并且可以同时高精度地确定立体结构。

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公开/公告号CA2471105A1

专利类型
公开/公告日2003-07-03

原文格式PDF
申请/专利权人 JAPAN SCIENCE AND TECHNOLOGY AGENCY;
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申请/专利号CA20022471105
发明设计人 KAINOSHO MASATSUNE;TERAUCHI TSUTOMU;
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申请日2002-12-19
分类号C07C229/08;C07B59/00;C07C229/24;C07C229/26;C07C229/36;C07C323/58;C12P21/00;G01N24/08;
国家 CA
入库时间 2022-08-21 23:58:09

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