The utility of the promoter/enhancer region (i.e. regulatory region)of the PSP94 gene in targeting expression of heterologous genes, tothe prostate gland was analyzed. Three breeding lines (i.e., line A,line B and line C) of the PSP-TGMAP model described herein wereestablished with constructs containing regulatory regions with orwithout additional exon/intron sequence of the PSP94 gene. Theseregulatory regions were operatively linked to the simian virus 40(SV40) T antigen (Tag, including both the large T and small tantigens). Histopathology evaluation of tumor grade, transgeneexpression, tumor responsiveness to androgen deprivation and tumormetastatic potential were analyzed and reported herein. This studyindicates that regulatory regions (e.g., the promoter/enhancer withexon/intron region) of the PSP94 gene efficiently target theexpression of heterologous genes to prostate tissue. Furthermore,the PSP-TGMAP model provides a valuable tool for the analysis ofprostate cancer development and for developing new gene therapyvector for the treatment of prostate cancer. Based on this study,mouse PSP94 promoter/enhancer region may be used in the generation ofa "shuttle vector" fit for prostate targeting in both mouse CaP modeland in human clinical trial.
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