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Characterization and quantification procedure soy protein by high-performance liquid chromatography in reverse phase of perfusion.

机译:高效液相色谱法在反相灌注过程中表征和定量大豆蛋白。

摘要

Process characterization and quantification of soy proteins by high-performance liquid chromatography reverse phase perfusion. The object of the invention is the development of an analytical method of high-performance liquid chromatography perfusion reverse phase to characterize and quantify soy protein products in commercial soy in short analysis times. The invention consists of a binary linear gradient in two steps with acetonitrile-water-trifluoroacetic acid to given flow versus time gradient (to maintain a constant volume gradient 9 ml), a temperature between 25 and 60 and detection 254 nm. Injection of the samples in aqueous solution is carried out directly in the chromatographic system.
机译:通过高效液相色谱反相灌注对大豆蛋白进行过程表征和定量。发明内容本发明的目的是开发一种高效液相色谱灌注反相分析方法,以在短分析时间内表征和定量商品大豆中的大豆蛋白产物。本发明由两步线性二元线性梯度组成,其中乙腈-水-三氟乙酸为给定的流量与时间的梯度关系(保持恒定的9 ml体积梯度),温度为25至60,检测波长为254 nm。样品在水溶液中的注射直接在色谱系统中进行。

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