首页> 外国专利> GENETICALLY MODIFIED MYXOBACTERIAL CELL FROM THE SPORANGIUM/ POLYANGIUM GROUP CONTAINING AN EXOGENOUS DNA INTEGRATED INTO THE GENOME VIA HOMOLOGOUS RECOMBINATION, PROCESS FOR ITS PREPARATION AND RECOMBINANT DNA WHICH MAKES POSSIBLE SAID INTEGRATION

GENETICALLY MODIFIED MYXOBACTERIAL CELL FROM THE SPORANGIUM/ POLYANGIUM GROUP CONTAINING AN EXOGENOUS DNA INTEGRATED INTO THE GENOME VIA HOMOLOGOUS RECOMBINATION, PROCESS FOR ITS PREPARATION AND RECOMBINANT DNA WHICH MAKES POSSIBLE SAID INTEGRATION

机译：含有来自通过同源重组整合到基因组中的外源DNA的孢子/ YANG基团的基因修饰的黏细菌细胞，其制备和重组DNA的过程（可能进行了整合）

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Process for the genetic manipulation of myxobacteria from the Sporangium/ Polyangium group, characterized in that: (a) genetic material of homologous or heterologous origin or a combination of 4064 י' באדר א' התשס" ג - February 12, 2003 genetic material of homologous and heterologous origin, which is homologous with or else at least essentially homologous with a corresponding region on the myxobacterial chromosome; or else (b) genetic material which naturally contains no sections which are homologous with or else at least essentially homologous with a corresponding region on the myxobacterial chromosome and is therefore artifically linked with the aid of rDNA techniques known per se to such homologous or else essentially homologous DNA sections, as homologous or else the essentially homologous DNA sections; is inserted into the myxobacterial cell and there integrated, via homologous recombination, at a site, which is accurately defined by reason of the homologies present between inserted DNA and DNA which is intrinsic to the bacteria, into the chromosome of said myxobacteria.

机译：用于孢子囊/多腺孢子菌群的黏细菌的遗传操作的方法，其特征在于：（a）同源或异源起源的遗传材料或4064י'באדרא'התשס”的组合-2003年2月12日异源，其与粘细菌染色体上的相应区域同源或至少基本上同源;或（b）天然不包含与该染色体上相应区域同源或至少基本同源的区段的遗传物质因此，借助于本身已知的rDNA技术，将其粘连到粘细菌染色体上，从而将这些同源或基本同源的DNA片段（例如同源或基本同源DNA的片段）插入粘细菌细胞中，并通过同源重组整合，在一个站点上，由于存在同质性而被准确定义b将插入的DNA和细菌固有的DNA插入所述粘细菌的染色体中。

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公开/公告号IL101097B

专利类型
公开/公告日2003-02-12

原文格式PDF
申请/专利权人 SYNGENTA PARTICIPATIONS AG;
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申请/专利号IL101097
发明设计人
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申请日1992-02-28
分类号C12N15/74;C12N1/21;C12N15/09;
国家 IL
入库时间 2022-08-22 00:02:46

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