首页> 外国专利> Isolated Nucleic Acid Sequence, Isolation, Gene Construct, amino acid sequences, Vector and isolated organism containing Molecule, Antibody, antisense Nucleic Acid, Process for preparing polyunsaturated fatty acids used, Oil, Lipids or fatty acids or FRA

Isolated Nucleic Acid Sequence, Isolation, Gene Construct, amino acid sequences, Vector and isolated organism containing Molecule, Antibody, antisense Nucleic Acid, Process for preparing polyunsaturated fatty acids used, Oil, Lipids or fatty acids or FRA

机译:分离的核酸序列,分离,基因构建,氨基酸序列,包含分子的载体和分离的生物,抗体,反义核酸,制备使用的多不饱和脂肪酸的方法,油,脂质或脂肪酸或FRA

摘要

A nucleic acid isolated from plants or algae that encodes a polypeptide to extend C16, C18, or C20 fatty acids and has at least two carbon atoms linked in the fatty acids. The peptide can extend C16, C18 or C20 fatty acids, and has at least two pairing links in the fatty molecules, which are selected from (a) nucleic acid sequences, represented by: id.de sec. 1, id.de sec. 3, id.de sec. 5, or id.de sec. 7; b) nucleic acid sequences, According to the degenerated genetic code from the sequences listed in Section 1, ibid., section 3, ibid., section 5.or ID. OF SEC No. 7; c) derivatives of the sequence represented ID. OF SEC No. 1, ID. OF SEC No. 3, ID. OF SEC No. 5, or ID. SEQ No. 7, which encode polypeptides of the amino acid sequence depicted in ID. OF SEC No. 2, ID. OF SEC No. 4, ID. OF SEC No. 6, or ID. SEQ No. 8 and representing at least 50% homology at the amino acid level, without significantly reducing the enzymatic action of the polypeptides. Gene construct, comprising the isolated nucleic acid described. The nucleic acid encoding a fatty acid biosynthesis gene may be selected from the delta group 19-,Delta 17Delta 15Delta 12Delta 9Delta 8Delta 6Delta 5delta 4- desaturase, the different hydroxylases, delta 12-acetylenesa, acyl-ACP-thioesrases, beta-ketoacyl-ACP-synthases or beta-ketoacyl-ACP-reductases. Amino acid sequence, which is encoded by said isolated nucleic acid sequence or the gene construct. Vector comprising the nucleic acid or the gene construct. Organism comprising at least one nucleic acid, the gene construct of or the vector described above. Antibody that specifically binds a polypeptide that is encoded by one of the nucleic acids described,Nucleic acid molecules, I understand a complementary nucleic acid sequence, prepared according to the description and procedures of PUFAs. Oil, fat, fatty acids, fatty acids or a part of them, through this process and constitute oil, fat or fatty acids in PUFAs, and come from transgenic plants. The use of crude oil, fat or fatty acid ingredients in feed, food, cosmetics or drugs, including antibodies, gene builders, molecules of nucleic acids, antibodies, known composers or dying persons, andSequence of amino acids and / or oils, fats and / or fatty acids, or part of them. A procedure for determining the death of an opponent or longasas.
机译:从植物或藻类分离的核酸,其编码多肽以延伸C16,C18或C20脂肪酸,并在脂肪酸中具有至少两个碳原子。该肽可以延伸C16,C18或C20脂肪酸,并且在脂肪分子中具有至少两个配对链,其选自(a)由id.de sec表示的核酸序列。 1,id.de sec。 3,id.de sec。 5,或id.de sec。 7; b)核酸序列,根据第1节,同上,第3节,同上,第5节或ID中列出的序列的简并遗传密码。 SEC第7号; c)代表ID的序列的衍生物。 OF SEC No. 1,ID。 OF SEC No. 3,ID。 SEC 5号或ID。 SEQ ID NO:7,其编码ID中描述的氨基酸序列的多肽。 OF SEC No. 2,ID。 OF SEC No. 4,ID。 OF SEC No. 6或ID。 SEQ ID NO:8,并且在氨基酸水平上表示至少50%的同源性,而没有显着降低多肽的酶促作用。基因构建体,包含所述的分离的核酸。编码脂肪酸生物合成基因的核酸可以选自δ组19-,δ17δ15δ12δ9δ8δ6δ5δ4-去饱和酶,不同的羟化酶,δ12-乙炔基,酰基-ACP-硫代擦除酶,β-酮酰基- ACP合酶或β-酮酰基-ACP还原酶。由所述分离的核酸序列或基因构建体编码的氨基酸序列。包含核酸或基因构建体的载体。包含至少一种上述核酸,其基因构建体或载体的生物。特异性结合由所述核酸之一编码的多肽的抗体,核酸分子,我理解根据PUFA的描述和程序制备的互补核酸序列。油,脂肪,脂肪酸,脂肪酸或它们的一部分,通过此过程构成PUFA中的油,脂肪或脂肪酸,它们来自转基因植物。在饲料,食品,化妆品或药物中使用原油,脂肪或脂肪酸成分,包括抗体,基因构建物,核酸分子,抗体,已知的作曲者或临终者以及氨基酸和/或油,脂肪和脂肪酸的序列/或脂肪酸,或其中的一部分。确定对手或longasas死亡的程序。

著录项

  • 公开/公告号AR030051A1

    专利类型

  • 公开/公告日2003-08-13

    原文格式PDF

  • 申请/专利权人 BASF AKTIENGESELLSCHAFT;

    申请/专利号AR2001P100619

  • 发明设计人

    申请日2001-02-09

  • 分类号C12N15/54;C12N15/82;C12N9/10;A01H5/00;C07K16/40;C12P7/64;C11B1/00;C11C1/00;G01N33/573;C12Q1/48;A61K38/45;

  • 国家 AR

  • 入库时间 2022-08-22 00:04:05

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