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Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion
Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion
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机译:利用熔解曲线和限制性内切酶消化分析单核苷酸多态性的方法
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摘要
A method for discriminating between two or more alleles which differ by only a single nucleotide is provided. This method is suitable for use in solution or on a solid phase and employs the use of restriction enzyme digestion of DNA in combination with Melting Curve Analysis. Also contemplated are specific markers to be included in melting curve analysis and melting curve genotyping experiments.
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