首页> 外国专利> METHOD FOR ESTIMATING GENOTYPE OF FERTILITY RESTORATIVE GENE LOCUS FOR ORYZA SATIVA BT-TYPE MALE STERILE CYTOPLASM

METHOD FOR ESTIMATING GENOTYPE OF FERTILITY RESTORATIVE GENE LOCUS FOR ORYZA SATIVA BT-TYPE MALE STERILE CYTOPLASM

机译:水稻BT型男性不育细胞生殖恢复基因位点基因型的估算方法。

摘要

PROBLEM TO BE SOLVED: To provide a method for detecting the fertility restorative gene (Rf-1 gene) of Oryza sativa individuals for RT-type male sterile cytoplasm having been utilized for raising hybrid rice. ;SOLUTION: This method comprises detecting the Rf-1 gene by making use of the fact that a plurality of PCR marker loci present in the proximity of the Rf-1 gene locus link with the Rf-1 gene locus. More specifically, this method comprises easily and accurately investigating the presence/absence of the Rf-1 gene and screening Rf-1 gene homo-type individual(s) by assaying the genotype of the plurality of PCR marker loci present in the proximity of the Rf-1 gene locus by making use of the fact that the Rf-1 gene locus lies between the new PCR marker loci, i.e. S12564 Tsp509I locus and C1361 MwoI locus, present on the Oryza sativa 10th chromosome.;COPYRIGHT: (C)2003,JPO
机译:解决的问题:提供一种用于检测已经用于饲养杂交水稻的RT型雄性不育细胞质的水稻个体的生育力恢复基因(Rf-1基因)的方法。解决方案:该方法包括利用存在于Rf-1基因位点附近的多个PCR标记基因座与Rf-1基因位点相连的事实来检测Rf-1基因。更具体地,该方法包括容易和准确地研究Rf-1基因的存在/不存在,并通过测定在Rf-1基因附近存在的多个PCR标记基因座的基因型来筛选Rf-1基因同型个体。利用Rf-1基因位点位于新的PCR标记基因座(即S12564 Tsp509I基因座和C1361 MwoI基因座)之间的事实,该基因存在于水稻10号染色体上;版权:(C)2003 ,日本特许厅

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