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Hybridization probes and primers for carrying out the process and methods for identifying an organism by comparative gene analysis.

机译:杂交探针和引物,用于进行通过比较基因分析鉴定生物的过程和方法。

摘要

And a hybridization probe method, and primers for the identification of organisms by comparative gene analysis, gene which is highly conserved, and coding and non-coding regions of homologues or pseudogenes (57) [Abstract] The present invention, / or is characterized in that the amplified regions functionally significant by using a PCR, then the genotyping and analyzed. Genes are highly conserved, the comparison of the coding and non-coding regions of homologues or pseudogenes, bind to DNA sequences that are highly conserved between species a single oligonucleotide pair is different, so that, for all species Amplification of the gene segments are the same becomes possible. Oligonucleotides, gene regions more or include one having a sequence differences most likely among different species. Determination of the gene sequence of the region of the highly polymorphic in subsequent reaction steps makes it possible to assign a particular species the gene sequences. In a preferred variant of the specific embodiment of the present invention, the oligonucleotide pair, the tumor suppressor gene PTEN / MMAC1 that are highly conserved, it possible to amplify the homologues and their pseudogene that was found.
机译:以及通过比较基因分析,高度保守的基因以及同源物或假基因的编码区和非编码区来鉴定生物的杂交探针方法和引物(57)[摘要]使用PCR扩增的区域具有重要的功能,然后进行基因分型并进行分析。基因是高度保守的,同源物或假基因的编码区和非编码区的比较,与物种之间高度保守的DNA序列结合,单个寡核苷酸对是不同的,因此,对于所有物种,基因片段的扩增是同样成为可能。寡核苷酸,基因区域更多或包括在不同物种之间最有可能具有序列差异的区域。通过在随后的反应步骤中确定高度多态性区域的基因序列,可以为特定物种指定基因序列。在本发明的具体实施方式的优选变体中,寡核苷酸对,高度保守的肿瘤抑制基因PTEN / MMAC1,可以扩增发现的同源物及其假基因。

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