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Methods and apparatus for in vitro detection and characterization of mental activity using analysis of repetitive electrical activity in neuronal samples

机译:使用神经元样本中的重复性电活动进行体外检测和表征精神活动的方法和设备

摘要

This relates to methods for the detection of psychoactive compounds in an in vitro neuronal tissue sample by detecting oscillations of extracellular voltage desirably before and after the introduction of a candidate sample onto an in vitro neuronal tissue sample and for devices useful in practicing the methods. Analysis of the extracellular voltage parameters leads to indication of the presence of psychoactive material in the candidate sample and information as to its pharmacological activity and/or composition. Further, it relates to a process of initiating and maintaining the presence of repetitive neuronal activity within the in vitro sample. Additionally, this includes a method for the stimulation of or initiation of repetitive neuronal activity, e.g., EEG, in an in vitro neuronal tissue sample by introducing a stimulating composition comprising compounds that facilitate or mimic the actions of acetylcholine, serotonin, or catecholamines, such as carbachol, or by subjecting the in vitro neuronal sample to electrical stimulation parameters.
机译:本发明涉及通过在将候选样品引入体外神经元组织样品上之前和之后理想地通过检测细胞外电压的振荡来检测体外神经元组织样品中的精神活性化合物的方法,以及用于实践该方法的装置。细胞外电压参数的分析导致候选样品中存在精神活性物质的指示以及有关其药理活性和/或组成的信息。此外,它涉及在体外样品中起始和维持重复神经元活性的存在的过程。另外,这包括通过引入刺激性组合物来刺激或引发体外神经元组织样品中的重复性神经元活性,例如EEG的方法,所述刺激性组合物包含促进或模拟乙酰胆碱,5-羟色胺或儿茶酚胺作用的化合物,例如如卡巴胆碱,或使体外神经元样品受到电刺激参数。

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