Labelled polyanionic dyes as a method to separate prion from normal forms of protein.

• 1. 一种标记病毒的荧光染料、制备方法和应用 [P] . 中国专利： CN113004712A . 2021-06-22
• 2. 一种网络入侵行为和正常行为的形式化描述方法 [P] . 中国专利： CN1477811A . 2004-02-25
• 3. Labelled polyanionic dyes as a method to separate prion from normal protein [P] . 英国专利： GB0027650D0 . 2000-12-27

  机译： 标记的聚阴离子染料作为将pr病毒与正常蛋白质分离的方法

• 4. Labelled polyanionic polyglycosides as a method to separate prion from normal forms of protein [P] . 英国专利： GB0128218D0 . 2002-01-16

  机译： 标记的聚阴离子多糖苷作为分离病毒和蛋白质正常形式的方法

• 5. Labelled polyanionic polyglycosides as a method to separate prion from normal forms of protein [P] . 英国专利： GB0027653D0 . 2000-12-27

  机译： 标记的聚阴离子多糖苷作为分离病毒和蛋白质正常形式的方法

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• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. 检测口蹄疫病毒的一种敏感方法——用生物素标记寡脱氧核苷酸和酪胺信号放大的原位杂交 [C] . 邵军军 . 中国畜牧兽医学会口蹄疫学分会第九次全国口蹄疫学术研讨会 . 2003
• 3. 基于适配体和抗体功能化纳米材料的疾病标记蛋白质分离分析新方法研究 [A] . 张学洋 . 2013