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Apparatus for spectroscopic analysis of metabolites in a culture medium, using nuclear magnetic resonance, has a twin tube channel structure through a reactor to give controlled physical/chemical parameters
Apparatus for spectroscopic analysis of metabolites in a culture medium, using nuclear magnetic resonance, has a twin tube channel structure through a reactor to give controlled physical/chemical parameters
Apparatus for in vivo spectroscopy analysis of metabolites in a culture medium, using nuclear magnetic resonance, has an assembly of tube channels with an inlet and an outlet to be linked to a reactor holding the culture medium. Apparatus (100) for in vivo spectroscopy analysis of metabolites in a culture medium, using nuclear magnetic resonance, has an assembly of tube channels (110,120) with an inlet (109) and an outlet (115) to be linked to a reactor holding the culture medium. A leading section (101) is formed into a coil and the following section (102) follows a straight path against the culture while certain nuclei are excited. The apparatus has a pump for the recirculation of the culture, between the tube inlet and the reactor, with a throughput of 1 ml/sec. The coiled path of the tube is over a given length so that, according to the pumped delivery, the movement time of the culture medium through the coils is 3-5 times the longitudinal relaxation time of the cellular nuclei in the culture medium. The straight path section of the tube has a measurement zone (103) for the nuclear magnetic resonance system, as the culture medium passes through it and back. The inlet and outlet tubes are of a supple plastics material, for connection to the interior of the reactor. A protective shrouding, of a plastics material, surrounds the leading coil section, to hold the reactor in the apparatus for the analysis using nuclear magnetic resonance. The leading section of the coiled tube path has a straight line entry (110a) to give the inlet, followed by a coiled section (111) to give the spiral path, leading into a straight path section for the return through the coils to the outlet. The inlet and outlet tube sections are identical. The second tube channel and the straight path tube section are in a concentric array. The straight path (112) of the first tube channel is offset and parallel to a section (113) of the second tube channel, linked by a curved base (112a). The two straight sections can be of a single tube, cut into two equal parts by a center wall. The tube channels are of glass, in one piece to give the two tube sections.
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机译：使用核磁共振对培养基中代谢物进行体内光谱分析的设备具有管通道组件，该管通道组件具有入口和出口，该入口和出口将与容纳培养基的反应器相连。用于利用核磁共振对培养基中代谢物进行体内光谱分析的装置（100），具有管通道（110,120）的组件，该管通道的入口（109）和出口（115）与装有反应器的反应器相连。培养基。前部区段（101）形成为线圈，并且随后的区段（102）沿着直的路径逆着培养物，同时激发某些核。该设备在管入口和反应器之间具有用于培养物再循环的泵，其处理量为1 ml / sec。管的盘绕路径在给定长度上，使得根据泵送的输送，培养基通过盘管的移动时间是培养基中细胞核的纵向弛豫时间的3-5倍。当培养基经过并返回时，管的笔直段具有用于核磁共振系统的测量区（103）。入口管和出口管由柔软的塑料制成，用于连接到反应器内部。由塑料材料制成的保护性覆盖物围绕前导线圈部分，以将反应堆保持在设备中，以利用核磁共振进行分析。盘管路径的前段有一条直线入口（110a）提供入口，然后是盘绕段（111）形成螺旋路径，通向一条直线段，以便通过盘管返回出口。 。入口和出口管部分相同。第二管通道和直线管段呈同心阵列。第一管通道的直线路径（112）与第二管通道的由弯曲的基部（112a）连接的部分（113）偏置并平行。两个直的部分可以是单个管，由中心壁切成两个相等的部分。管通道是玻璃制成的，可以分成两个管段。
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