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/11 METHOD TO IMPROVE THE STABILITY AND BROADEN THE PH RANGE OF FAMILY G/11 XYLANASES

机译:/ 11提高稳定性和扩大家族G / 11木聚糖酶PH值的方法

摘要

The present invention relates to protein engineering, and in particular to lineage G / 11 xylanase and genes encoding such enzymes. In particular, the present invention relates to the Trichoderma reesei XYNII gene encoding endo-1,4-β-xylanase (EC 3.2.1.8). The present invention discloses a method of using site-specific mutagenesis to enhance the properties of an enzyme to meet the industrial conditions of the industry in which it is used. Protein engineering can be used to enhance the thermal activity and thermal stability of xylanase and to extend their pH range.
机译:本发明涉及蛋白质工程,特别是涉及谱系G / 11木聚糖酶和编码这种酶的基因。特别地,本发明涉及编码内切1,4-β-木聚糖酶(EC 3.2.1.8)的里氏木霉XYNII 基因。本发明公开了一种使用位点特异性诱变来增强酶的性质以满足使用它的工业的工业条件的方法。蛋白质工程可用于增强木聚糖酶的热活性和热稳定性,并扩展其pH范围。

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