首页>
外国专利>
Method for proliferating haematopoietic cells in vitro in the absence of exogenous cytokines
Method for proliferating haematopoietic cells in vitro in the absence of exogenous cytokines
展开▼
机译:在不存在外源细胞因子的情况下体外增殖造血细胞的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
A method for stimulating hematopoeitic cells in vitro comprising: (1) contacting the hematopoietic cells with a sufficient amount of an inhibitor of a dipeptidylpeptidase type IV (DPIV) to increase the number of said hematopoietic cells and/or the differentation state of said hematopoietic cells relative to the number and differentiation of hematopoietic cells that are present in a control culture that is not contacted with the inhibitor but is otherwise subjected to the same culture conditions as the hematopoietic cells which are cultured in the presence of the inhibitor; and (2) culturing the hematopoietic cells in the presence of the inhibitor and in the absence of exogenous cytokine under conditions and for a time sufficient to increase the number of hematopoietic cells and/or the differentiation of the hematopoietic cells relative to the number of hematopoietic cells that were present in the control culture. The hematopoietic cells may be selected from hematopoietic stem cells, primordial stem cells, early progenitor cells, CD34+ cells, early lineage cells of the mesenchymal, myeloid, lymphoid and erythroid lineages, bone marrow cells, blood cells, umbilical cord blood cells, stromal cells, and hematopoietic precursor cells. An apparatus comprising a container; and an inhibitor of DPIV contained therein or attached thereto and composition comprising a magnetic particle with an inhibitor of DPIV attached thereto.
展开▼