首页> 外国专利> A procedure to provide a cell eukaryotic DHFR negative, to introduce a procedure of a heterologous DHFR Gene in a eukaryotic cell.A Vector for homologous recombination and a eukaryotic Cell, preferably a human cell which cannot give rise to an entire organism.

A procedure to provide a cell eukaryotic DHFR negative, to introduce a procedure of a heterologous DHFR Gene in a eukaryotic cell.A Vector for homologous recombination and a eukaryotic Cell, preferably a human cell which cannot give rise to an entire organism.

机译：提供细胞真核DHFR阴性的程序，以在真核细胞中引入异源DHFR基因的程序。用于同源重组的载体和真核细胞，优选不能产生整个生物体的人细胞。

页面导航

摘要
著录项
相似文献

摘要

A procedure for the provision of a cell eukaryotic DHFR negativeIt comprises the steps of: (a) the cell is transfected with a First vector comprising (i) at least one target Sequence for a site specific Recombinase.(ii) DNA sequences homologous to a sequence of u00e1cidonucleico DHFR present endogenously in the cell that Flank the sequence (i), to enable a homologous recombination.And (iii) in a given case, genmarcador of seleccionpositiva and, if given a negative Selection Marker Gene.(b) The Transfected Cell Was cultivated under the conditions cualesproduce a homologous recombination vector, and (c) the cell obtained according to step (b) is recovered.The presentetambiu00e9n refers to a procedure for introduction of a DHFR Gene in the heterologous Cell obtained by this procedure.A Vector for carrying out such procedures and a cu00e9lulaeucariotica obtained by these procedures.

机译：提供细胞真核DHFR阴性的方法包括以下步骤：（a）用第一载体转染细胞，该第一载体包含（i）至少一个位点特异性重组酶的靶序列。（ii）与DNA同源的DNA序列。 cidonucleico DHFR序列内源地存在于序列的侧面（i），以实现同源重组;以及（iii）在特定情况下为seleccionpositiva genmarcador，如果给定负选择标记基因，则为（b）。在Cuales产生同源重组载体的条件下培养转染的细胞，并且（c）回收根据步骤（b）获得的细胞。介绍一种将DHFR基因导入由此获得的异源细胞的方法。用于执行此类过程的载体，以及通过这些过程获得的结果。

著录项

公开/公告号AR020893A2

专利类型
公开/公告日2002-06-05

原文格式PDF
申请/专利权人 ROCHE DIAGNOSTICS GMBH;
展开▼

申请/专利号AR1999P105097
发明设计人
展开▼

申请日1999-10-08
分类号C12N15/85;C12N5/10;C12N15/65;C12N15/90;
国家 AR
入库时间 2022-08-22 00:46:29

相似文献

专利
外文文献
中文文献