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Isolation and characterization of a rat epidermal growth factor related protein

机译:大鼠表皮生长因子相关蛋白的分离与鉴定

摘要

A cDNA fragment clone of 1583 base pairs with 90-95% sequence homology to the mouse EGFR and a truncated rat EGFR is described. The isolated cDNA is termed ERRP (EFG-Receptor Related Protein). In Northern-blot analysis with poly A+ RNA from different rat tissues, ERRP cDNA hybridized strongly to a mRNA transcript of about 1.8 kb. Maximal expression was noted in the small intestine, followed by colon, liver, gastric mucosa and other tissues. Transfection of ERRP cDNA in HCT-116 cells, a colon cancer cell line, markedly inhibits proliferation in monolayer and in soft agar and attenuates EGF-R tyrosine kinase activity. Transfection of ERRP in HCT-116 also delays tumor growth in SCID mice injected with these cells compared to the vector-transfector control cells. On the other hand, proliferation of the vector-transfected control, but not ERRP transfected HCT cells could be stimulated by TGF-&agr; (10−10M and 10−9M). The ERRP cDNA represents a new member of the EGFR gene family, and its protein product plays a key role in modulating the function of EGFR.
机译:描述了1583个碱基对的cDNA片段克隆,与小鼠EGFR和截短的大鼠EGFR具有90-95%的序列同源性。分离的cDNA被称为ERRP(EFG受体相关蛋白)。在使用poly A&plus进行的Northern印迹分析中;来自不同大鼠组织的RNA,ERRP cDNA与约1.8 kb的mRNA转录物强烈杂交。在小肠中发现最大的表达,其次是结肠,肝,胃粘膜和其他组织。 ERRP cDNA在结肠癌细胞系HCT-116细胞中的转染显着抑制单层和软琼脂中的增殖,并减弱EGF-R酪氨酸激酶活性。与载体转染对照细胞相比,HCT-116中ERRP的转染也延迟了注射了这些细胞的SCID小鼠的肿瘤生长。另一方面,TGF-α可刺激载体转染的对照而不是ERRP转染的HCT细胞的增殖。 (10 − 10 M和10 − 9 M)。 ERRP cDNA代表EGFR基因家族的新成员,其蛋白质产物在调节EGFR功能中起关键作用。

著录项

  • 公开/公告号US6399743B1

    专利类型

  • 公开/公告日2002-06-04

    原文格式PDF

  • 申请/专利权人 DEPT. OF VETERANS AFFAIRS;

    申请/专利号US20000570454

  • 发明设计人 ADHIP P. N. MAJUMDAR;

    申请日2000-05-12

  • 分类号C07K140/00;

  • 国家 US

  • 入库时间 2022-08-22 00:46:55

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