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Method for isolation of xylanase gene sequences from soil DNA, compositions useful in such method and compositions obtained thereby
Method for isolation of xylanase gene sequences from soil DNA, compositions useful in such method and compositions obtained thereby
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机译：从土壤DNA中分离木聚糖酶基因序列的方法，可用于该方法的组合物和由此获得的组合物
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摘要
Xylanase DNA is recovered from soil by PCR amplification using degenerate primers. Because of the complexity of the soil samples, it is likely that the recovered product will include more than one species of polynucleotide. These recovered copies may be cloned into a host organism to produce additional copies of each individual species prior to characterization by sequencing. Recovered DNA which is found to vary from known xylanases can be used in several ways to facilitate production of novel xylanases for industrial application. First, the recovered DNA, or probes corresponding to portions thereof, can be used as a probe to screen DNA libraries and recover intact xylanase genes including the unique regions of the recovered DNA. Second, the recovered DNA or polynucleotides corresponding to portions thereof, can be inserted into a known xylanase gene to produce a recombinant xylanase gene with the sequence variations of the recovered DNA.
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