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Low temperature revelation type kichinaze gene and its isolated method

机译:低温揭示型菊苣基因及其分离方法

摘要

A winter wheat derived chitinase cDNA characterised in that the cDNA encodes a protein with chitinase activity at low temperatures. The chitinase polypeptides may have an amino acid sequence corresponding to SEQ ID NOS: 1, 2 or 3. Also claimed is a method cloning such cDNAs using a standard PCR based protocol with specific primers (SEQ ID NOS:4 and 5) to generate fragments for library screening. Transgenic plants transformed with a winter wheat derived chitinase cDNA are claimed.
机译:冬小麦衍生的几丁质酶cDNA,其特征在于该cDNA编码在低温下具有几丁质酶活性的蛋白质。几丁质酶多肽可具有对应于SEQ ID NOS:1、2或3的氨基酸序列。还要求保护的方法是使用基于PCR的标准协议与特定引物(SEQ ID NOS:4和5)克隆此类cDNA以产生片段的方法。用于图书馆筛选。要求保护用冬小麦衍生的几丁质酶cDNA转化的转基因植物。

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