首页> 外国专利> PCR PCR Multiplex polymerase chain reaction-based method for fast detecting of pathological Escherichia Coil and PCR primers used for this method

PCR PCR Multiplex polymerase chain reaction-based method for fast detecting of pathological Escherichia Coil and PCR primers used for this method

机译：基于PCR PCR多重聚合酶链反应的病理大肠杆菌快速检测方法以及该方法所用的PCR引物

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摘要

The present invention relates to a multiplex polymerase chain reaction-based method that can quickly identify enterotoxin generating genes of pathogenic E. coli causing diarrhea in livestock, and more particularly, known dipyrotoxin (LT). : PCR primer for heat-stable enterotoxin generating gene which can be applied to one PCR reaction simultaneously with PCR primer for heat-labile enterotoxin generating gene. The present invention relates to a multiplexer PCR technique for simultaneous detection of E. coli enterotoxin gene.;The PCR primers for the heat resistant toxin (STIa) generating gene provided by the present invention can be simultaneously applied to one PCR reaction together with the PCR primers for the known LT producing gene, so that the pathogenic factors of Escherichia coli are performed by one PCR. Phosphorus two genes can be detected simultaneously and E. coli diarrhea can be diagnosed quickly and accurately without undergoing various biochemical and serological tests.

机译：基于多重聚合酶链反应的方法技术领域本发明涉及一种基于多重聚合酶链反应的方法，该方法可以快速鉴定引起家畜腹泻的致病性大肠杆菌的肠毒素产生基因，尤其是已知的双丙酮毒素（LT）。：用于产生热稳定肠毒素的基因的PCR引物，可与用于产生热不稳定肠毒素的基因的PCR引物同时用于一个PCR反应。本发明涉及一种同时检测大肠杆菌肠毒素基因的多重PCR技术。本发明提供的产生耐热毒素（STIa）基因的PCR引物可以与PCR同时用于一个PCR反应。已知的LT产生基因的引物，因此大肠杆菌的致病因子可通过一次PCR进行。可以同时检测到两个磷基因，并且可以快速准确地诊断出大肠杆菌的腹泻，而无需进行各种生化和血清学检测。

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公开/公告号KR100293571B1

专利类型
公开/公告日2001-09-17

原文格式PDF
申请/专利权人 대한민국;
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申请/专利号KR19980024503
发明设计人 우승룡;임숙경;이희수;김종만;김종염;
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申请日1998-06-27
分类号C12Q1/68;
国家 KR
入库时间 2022-08-22 01:12:18

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