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REGULATING ORGANELLE HOMEOSTASIS IN A CELL FACTORY

机译:调节细胞工厂内的有机平衡

摘要

The present invention relates to a method for modulating the production of secondary metabolites in the fungus iPenicillium chrysogenum/i by influencing the homeostasis of organelles by modulating a least one gene which is directly related to organelle homeostasis in this fungus. Preferably, the organelle homeostasis is modulated by genetically engineering at least one gene encoding a protein which is responsible for organelle biogenesis (the expression and/or activity of which is to be increased) and/or at least one gene encoding a protein which is responsible for organelle degeneration (the expression and/or activity of which is to be decreased). Genes involved in organelle biosynthesis in iP. chrysogenum/i are exemplified by the so-called ipex11/i gene. Genes involved in organelle degradation are exemplified by the so-called ivps15/i and ipdd1/i genes.
机译:本发明涉及一种通过调节至少一种与真菌中的细胞器稳态直接相关的基因来影响细胞器的稳态来调节真菌在产黄青霉中的次生代谢产物的方法。优选地,通过基因工程改造至少一种编码负责细胞器生物发生的蛋白质(其表达和/或活性将增加的蛋白质)的基因和/或至少一种编码负责蛋白质的基因的基因来调节细胞器的稳态。用于细胞器变性(其表达和/或活性将降低)。 P中参与细胞器生物合成的基因。 chrysogenum 以所谓的 pex11 基因为例。参与细胞器降解的基因有所谓的 vps15 和 pdd1 基因。

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