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Method of quick determination of an organ state based on detection one or more isoenzymes of glutathione S-transferase and apparatus for making the same
Method of quick determination of an organ state based on detection one or more isoenzymes of glutathione S-transferase and apparatus for making the same
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机译:基于检测谷胱甘肽S-转移酶的一种或多种同功酶快速确定器官状态的方法及其制备装置
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摘要
The invented method comprises contacting some biological liquids, such as bile, plasma, serum and urine, with an antibody anti-GST, labeled with particles such as gold particles and specific for the isoenzyme to be tested, whereby the specific anti-GST antibody must exhibit such sensitivity that makes it possible to detect picomolar concentrations of the isoenzyme under test. Complex being formed, i.e. "particle labeled antibody-isoenzyme" is caught on the immobilized antibody under generation of a detectable signal. The proposed method is suitable for determining damage of organs due to immunological or toxicological damage and for monitoring the state of transplanted organs, such as livers or kidneys.
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