首页>
外国专利>
Cell line of imported human keratinocytes, methods of testing the effect of a test tumor cell modulating agent and for evaluating test compounds for human toxicity, and organotypic culture.
Cell line of imported human keratinocytes, methods of testing the effect of a test tumor cell modulating agent and for evaluating test compounds for human toxicity, and organotypic culture.
"CELLULAR LINING OF IMMORTALIZED HUMAN KERATINOCYTES, METHODS OF TESTING THE EFFECT OF A TUMOR TESTING MODULE AGENT AND TO EVALUATE TESTING COMPOUNTS FOR HUMAN TOXICITY, AND, ORGANOTIC CULTURE." a cell line of spontaneously immortalized human keratinocytes is presented. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of testing the effect of a test tumor cell modulating agent is presented. The method comprises the steps of obtaining a culture of stratified human squamous epithelial cells, in which the culture comprises human malignant squamous epithelial cells and spontaneously immortalized human keratinocytes, in which the culture forms a reconstituted epidermis. The epidermis is then treated with a test tumor cell modulating agent and the growth of malignant cells within the epidermis is evaluated.
展开▼
