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Manipulated by Metabolic Engineering a cell; a method for producing such a cell; a method for producing a Biochemical substance derived deoxalacetato and isolated Nucleic Acid Fragment.P. fluorescens
Manipulated by Metabolic Engineering a cell; a method for producing such a cell; a method for producing a Biochemical substance derived deoxalacetato and isolated Nucleic Acid Fragment.P. fluorescens
A cell manipulated by Metabolic Engineering comprising pyruvate carboxylase in the cell where manipulated by Metabolic Engineering on - expresapiruvato - carboxylase.The method to obtain this Cell includes the introduction of a Nucleic Acid fragment and / or strange that encode unapiruvato - carboxylase inside a host cell.So what happens to an overproduction of pyruvate carboxylase in the cell.Alternatively, a cell's DNA that endogenously Express a pyruvate carboxylase can be mutated to alter - Native pyruvate carboxylase Gene transcription.Retail manner that causes an overproduction of pyruvate carboxylase in the cell.Generally, the DNA fragment encoding a pyruvate carboxilasaderivado - an organism that endogenously express the pyruvate carboxylase, as etli Rhizobium and Pseudomonas fluorescens.Optionally, the cu00e9lulamanipulada by Metabolic Engineering over expressed fosfoenol pyruvate carboxylase (PEP) in addition of pyruvate carboxylase.Preferably, the cell is a cell manipuladapor Metabolic Engineering of C. glutamicum, e.Coli, Brevibacterium flavum or Brevibacterium lactofermentum expresses a carboxilasaheterologa pyruvate.A method for producing a substance derived from oxaloacetate Biochemistry includes providing a cell that produce this substance Biochemistry; Transforming the cell with a Nucleic Acid fragmentOr,Which contains a Nucleotide sequence that encodes an enzyme whose activity depiruvato - carboxylase; express the Enzyme in the cell to cause increased production of SubstitutionThe Biochemistry; and then isolate the substance Chem panelon the cell. A fragment of nucleic acid aisladode p.Fluorescens useful in the method for producing the cell includes a sequence quecodifica manipulated a pyruvate carboxylase.
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