Improved methods of animal husbandry methods and techniques to increase the microbial population of the rumen

• 1. 改善医疗技术装置利用负荷的方法和系统及医疗技术装置 [P] . 中国专利： CN1252628C . 2006.04.19
• 2. 改善医疗技术装置利用负荷的方法和系统及医疗技术装置 [P] . 中国专利： CN1379360A . 2002-11-13
• 3. A method for increasing the microbial population in the rumen the ruminant and to improve the zootechnical power of these animals [P] . 德国专利： DE69209858T2 . 1996-10-24

  机译： 一种增加瘤胃中反刍动物微生物种群并提高这些动物的动物技术能力的方法

• 4. To increase the microbial population of the rumen of the ruminant animals and in order to improve the zootechnical performance levels of these animals. [P] . 法国专利： FR2671698B1 . 1994-05-20

  机译： 增加反刍动物瘤胃的微生物种群，并提高这些动物的动物技术水平。

• 5. PROCESS FOR INCREASING THE MICROBIAL POPULATION OF RUMEN ANIMALS IN RUMINANTS AND FOR IMPROVING THE ZOOTECHNIC PERFORMANCE OF THESE ANIMALS. [P] . 法国专利： FR2671698A1 . 1992-07-24

  机译： 增加反刍动物中瘤胃动物的微生物种群并提高这些动物的动物技术性能的方法。

• 1. Effects of Neutral Detergent Soluble Fiber and Sucrose Supplementation on Ruminal Fermentation, Microbial Synthesis, and Populations of Ruminal Cellulolytic Bacteria Using the Rumen Simulation Technique （RUSITEC） [J] . ZHAO Xiang-hui, LIU Chan-juan, LI Chao-yun, 农业科学学报：英文版 . 2013,第008期
• 2. Effects of Neutral Detergent Soluble Fiber and Sucrose Supplementation on Ruminal Fermentation, Microbial Synthesis, and Populations of Ruminal Cellulolytic Bacteria Using the Rumen Simulation Technique (RUSITEC) [J] . ZHAO Xiang-hui, LIU Chan-juan, LI Chao-yun, 农业科学学报（英文版） . 2013,第008期
• 3. Comparisons of improved genomic predictions generated by different imputation methods for genotyping by sequencing data in livestock populations [J] . Xiao Wang, Guosheng Su, Dan Hao, 畜牧与生物技术杂志：英文版 . 2020,第002期
• 4. Improving membrane integrity monitoring indirect methods to reduce plant downtime and increase microbial removal credit [C] . Gil F. Crozes, Sandeep Sethi, Baoxia Mi, International congress on membranes and membrane processes . 2002
• 5. Effects of Iso-Alpha-Acids on Rumen Fermentation and Comparison of Microbial Populations Between Rumens and Continuous Culture Fermenters [D] . Salfer, Isaac James. 2015
• 6. Effect of DNA Extraction Methods and Sampling Techniques on the Apparent Structure of Cow and Sheep Rumen Microbial Communities [O] . Gemma Henderson, Faith Cox, Sandra Kittelmann, -1
• 7. Effect of DNA Extraction Methods and Sampling Techniques on the Apparent Structure of Cow and Sheep Rumen Microbial Communities [O] . Gemma Henderson, Faith Cox, Ra Kittelmann, 2015
• 8. Methodology for Assessing Population and Ecosystem Level Effects Related to Intake of Cooling Waters. Volume 1:'Handbook of Methods: Population Level Techniques [R] . Ronald A. Alevras, C. Braxton Dew, Ellen K. Pikitch 1980

• 1. Improving Loading Facilities and Methods to Minimize Stress on Animals during Transport from Farm to Abattoir [J] . Girma Gebresenbet ,Techane Bosona ,Mulualem Feleke . 农业科学与技术：A . 2012,第006期
• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 3. 稀释平板技术应用于红树林区土壤微生物数量研究的方法学改进 [C] . 张瑜斌 ,林鹏 ,邓爱英 . 第五届微生物生态学术研讨会 . 2003
• 4. 一种改善沉淀池运行效果的技术措施及污泥膨胀控制方法 [A] . 杨振鼎 . 2016